Can't check specificity in some genome[KeyError] about primerserver2

[billzt]

Hello, can you show me what's your input and parameters? (If the file is big, give a part of sequence is OK)

[JianMingLuo]

Hello, can you show me what's your input and parameters? (If the file is big, give a part of sequence is OK)

i use it on web UI,i only input the geneID,the region type is Include Region

the genome fatsa is: http://citrus.hzau.edu.cn/data/Genome_info/RL.v1.0/RL.v1.0.CDS.fa

[billzt]

I download the template fasta (in fact it is CDS, not genome) and run command like this (input is your iD: Cm000010.1):

primertool full input RL.v1.0.CDS.fa --type SEQUENCE_INCLUDED_REGION -a --checking-size-max 300 --Tm-diff 10 --primer-num-return 10

There is no error

[billzt]

Please be note that genome has to be indexed by samtools first, and make sure the index file is correct

[JianMingLuo]

Please be note that genome has to be indexed by samtools first, and make sure the index file is correct

It still can't work on my computer.I ensure the genome has benn indexed .I have 9 genome ,only 2 of them cant work.

[billzt]

How about running in the command line?

[JianMingLuo]

primertool full 1.txt --type SEQUENCE_INCLUDED_REGION -a --checking-size-max 300 --Tm-diff 10 --primer-num-return 10 0012_Citrus_Medica_V1.0_HZAU_CDS.fasta

Here is my cmd,it cant work on web UI too.