opening CSQ files about thermimagej

[oseynnes]

I have used Fiji for a long time, it was a stupid mistake. Thanks again for your support and for sharing this great plugin. Le 12 mars 2020 à 16:29, Glenn Tattersall <[email protected]> a écrit :  Aha! FIJI is Just ImageJ. The circular acronym doesn't really help! Glad you figured it out, because I would not have known. I do need to add some error checking for file/folder executions and some more informative error messages. Eventually I'll have that up in the install instructions. Thanks for filing the issue. At least I'll know what trips users up. — You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub<#3 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AKUEPUIEYHGYXOMN4SK6ETLRHD5UDANCNFSM4LGGWUDQ>.

[gtatters]

are you getting this error running the sample csq I provide? https://github.com/gtatters/ThermImageJ/blob/master/SampleFiles.zip

It is possible that my perl split script needs adjusting for your file type. I could take a look at a sample file of yours if you are able to send it to me?

[oseynnes]

Thank you. Yes, I get the same error message. Could it come from an installation problem? I am happy to send you my file if you want. At this address? On 12 Mar 2020, at 11:45, Glenn Tattersall <[email protected]<mailto:[email protected]>> wrote: are you getting this error running the sample csq I provide? https://github.com/gtatters/ThermImageJ/blob/master/SampleFiles.zip It is possible that my perl split script needs adjusting for your file type. I could take a look at a sample file of yours if you are able to send it to me? — You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub<#3 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AKUEPUMCX3HSGIE7GHY7ZOTRHC4NBANCNFSM4LGGWUDQ>.

[gtatters]

ok. so, that suggests an install problem, so sending me file might not help yet. Can you verify whether the split.pl script was copied to your FIJI/scripts folder, according to the (brief) instructions.

[oseynnes]

Yes, the split.pl file is in Fiji/scripts. Perhaps something that I need to change in the split script? On 12 Mar 2020, at 11:57, Glenn Tattersall <[email protected]<mailto:[email protected]>> wrote: ok. so, that suggests an install problem, so sending me file might not help yet. Can you verify whether the split.pl script was copied to your FIJI/scripts folder, according to the (brief) instructions. — You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub<#3 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AKUEPUIVPX3A7AZ7Q66HYR3RHC52TANCNFSM4LGGWUDQ>.

[gtatters]

and the installation checks? did they produce errors? I'd need to know that exiftool, perl and ffmpeg are installed properly.

[gtatters]

I haven't tested on Catalina yet (I've been delaying the update to get bugs out of the os), but I doubt that is the issue. Unfortunately, I won't update to Catalina until the end of semester to avoid sudden loss of function during teaching. Hopefully I can diagnose the problem one error at a time.

[gtatters]

Hmm..i just noticed in your error message that FIJI seems to think that the split.pl is in a different subfolder than Fiji/scripts. But on line 57 of the toolset, I know I scan for where your ImageJ folder is. Can you confirm the precise path location of your FIJI install? I would advise you place FIJI in a folder that you have read+write permission. Usually on a Mac, FIJI goes directly in the Application folder:

i.e.
/Applications/FIJI

thus, the scripts would be:
/Applications/FIJI/scripts

I don't know if this will affect functionality or not.

[oseynnes]

It now works! I had made a mistake for the line "var perlscriptpath=getDirectory("imageJ") + "scripts" + File.separator;”. I thought that I should replace “imageJ” by “Fiji”. I have now set the directory address with “imageJ” and it works. Thank you for your help. On 12 Mar 2020, at 13:35, Glenn Tattersall <[email protected]<mailto:[email protected]>> wrote: Hmm..i just noticed in your error message that FIJI seems to think that the split.pl is in a different subfolder than Fiji/scripts. But on line 57 of the toolset, I know I scan for where your ImageJ folder is. Can you confirm the precise path location of your FIJI install? I would advise you place FIJI in a folder that you have read+write permission. Usually on a Mac, FIJI goes directly in the Application folder: i.e. /Applications/FIJI thus, the scripts would be: /Applications/FIJI/scripts I don't know if this will affect functionality or not. — You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub<#3 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AKUEPULNDBXFCOPSJLVV2ELRHDJJJANCNFSM4LGGWUDQ>.

[gtatters]

Aha! FIJI is Just ImageJ. The circular acronym doesn't really help! Glad you figured it out, because I would not have known.

I do need to add some error checking for file/folder executions and some more informative error messages. Eventually I'll have that up in the install instructions.

Thanks for filing the issue. At least I'll know what trips users up.

[oseynnes]

On a separate note. I am dealing with moving objects (human movement) and it would be nice to be able to run the analysis on distinct ROIs associated with each image of the sequence (currently it seems that the same ROI is applied to all images). Another nice feature would have been the possibility to automatically save ROIs based on temperature threshold. This would be useful for the above point but also to analyse sub-areas of changing temperature. I did not fill these in as request for new features on Github as they have nothing to do with the function of the plugin as it is designed, but just in case ;) On 12 Mar 2020, at 16:29, Glenn Tattersall <[email protected]<mailto:[email protected]>> wrote: Aha! FIJI is Just ImageJ. The circular acronym doesn't really help! Glad you figured it out, because I would not have known. I do need to add some error checking for file/folder executions and some more informative error messages. Eventually I'll have that up in the install instructions. Thanks for filing the issue. At least I'll know what trips users up. — You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub<#3 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AKUEPUIEYHGYXOMN4SK6ETLRHD5UDANCNFSM4LGGWUDQ>.

[gtatters]

I would like that as well! With colleagues, outside of this software, we are trying to get Machine learning roi coding working, but it won't likely be part of ThermimageJ. Early days yet for that.

But at least you can use ThermimageJ to extract or convert CSQ files into a format where you might do that in another environment.

As for ROI saving...that seems like some kind of feature that ImageJ might already have, but I know what you mean. That is probably beyond my skills for the time being. You could probably use ImageJ masking features to remove all parts of the image space that are below or above your threshold temperature. I am less likely to develop those features, much as they would be nice to have!

[gtatters]

in terms of a moving ROI...there are Tracker or Tracking plugins that you might be able to get to work that are available in FIJI. Once you have the ROIs registered, I think those plugins allow you to extract certain types of info like mean, max or median intensity scores (which would be temperature if you ran the conversions).

[oseynnes]

Yes, I am looking into this. Thank you again. On 12 Mar 2020, at 19:40, Glenn Tattersall <[email protected]<mailto:[email protected]>> wrote: in terms of a moving ROI...there are Tracker or Tracking plugins that you might be able to get to work that are available in FIJI. Once you have the ROIs registered, I think those plugins allow you to extract certain types of info like mean, max or median intensity scores (which would be temperature if you ran the conversions). — You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub<#3 (comment)>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/AKUEPUO4YMQ7ZP2PJQ7XY4TRHEUCNANCNFSM4LGGWUDQ>.