Comments (3)
I wanted to run 18S data using the current version (pema v.2.1.3) and with the vsearch algorithm.
So, at the "GENE - dependent parameters" I wrote
gene gene_18S
This was my parameter setting
parameters.txt
However, the analysis was reaching step 4 (mergine) and an error was coming up
`
Merging step by SPAdes is completed
Marker gene under study 18S.
Fatal error: /home/modules/initialize.bds, line 193, pos 18. Map 'params' does not have key 'clusteringAlgoFor16S_18SrRNA'.
pema_latest.bds, line 95 : buildDirectories(paramsSpadesMerging, globalVars)
initialize.bds, line 158 : string buildDirectories(string{} params, string{} globalVars){
initialize.bds, line 162 : if ( params{'gene'} == 'gene_COI' ) {
initialize.bds, line 175 : } else if ( params{'gene'} == 'gene_16S' ) {
initialize.bds, line 188 : } else if ( params{'gene'} == 'gene_18S' ) {
initialize.bds, line 193 : if ( params{'clusteringAlgoFor16S_18SrRNA'} == 'algo_Swarm' ) {
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 10 / 0, nodes: 1422 -> 8178 -> 8179 -> 2295 -> 2303 -> 2356 -> 2409 -> 2415 -> 2432 -> 2433
Node Id : 2434
bdsNode Id : 2433
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 9 / 0, nodes: 1422 -> 8178 -> 8179 -> 2295 -> 2303 -> 2356 -> 2409 -> 2415 -> 2432
Node Id : 2433
bdsNode Id : 2432
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 8 / 0, nodes: 1422 -> 8178 -> 8179 -> 2295 -> 2303 -> 2356 -> 2409 -> 2415
Node Id : 2432
bdsNode Id : 2415
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 7 / 0, nodes: 1422 -> 8178 -> 8179 -> 2295 -> 2303 -> 2356 -> 2409
Node Id : 2415
bdsNode Id : 2409
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 6 / 0, nodes: 1422 -> 8178 -> 8179 -> 2295 -> 2303 -> 2356
Node Id : 2409
bdsNode Id : 2356
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 5 / 0, nodes: 1422 -> 8178 -> 8179 -> 2295 -> 2303
Node Id : 2356
bdsNode Id : 2303
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 4 / 0, nodes: 1422 -> 8178 -> 8179 -> 2295
Node Id : 2303
bdsNode Id : 2295
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 3 / 0, nodes: 1422 -> 8178 -> 8179
Node Id : 2295
bdsNode Id : 8179
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 2 / 0, nodes: 1422 -> 8178
Node Id : 8179
bdsNode Id : 8178
ProgramCounter.pop(100): Node ID does not match!
PC : PC: size 1 / 0, nodes: 1422
Node Id : 8178
bdsNode Id : 1422
`
But then I tricked the parameters and instead of 18S, I wrote
gene gene_16S
And everything worked fine.
from pema.
The issue has been resolved simultaneously with the #24 issue.
from pema.
In addition, a sannity_check
folder is now available.
Input data and parameters files support cross checking each and every marker gene and software combination.
from pema.
Related Issues (20)
- add remove_singletons option in OTU pipeline HOT 4
- Error when unzipping using a checkpoing HOT 1
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- extended final table HOT 1
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- Unexpected Singletons in Final Table despite removeSingletons set to "Yes" HOT 5
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- Needed updates on "PEMA's output files.md" HOT 2
- time consuming preprocessing
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- pandaseq error "Something is wrong with this ID" HOT 17
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from pema.