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wynstep avatar wynstep commented on August 13, 2024

@heathsc

This is the error that recurrently appears when it fails:
----------- Methylation Calling -------- : Reference : /usr/local/bin/ToTEM//src/ucsc.hg19.gemBS.ref : Species : Homo sapiens : Right Trim : 0 : Left Trim : 0 : Chromosomes : ['chr1', 'chr2', 'chr3', 'chr4', 'chr5', 'chr6', 'chr7', 'chrX', 'chr8', 'chr9', 'chr10', 'chr11', 'chr12', 'chr13', 'chr14', 'chr15', 'chr16', 'chr17', 'chr18', 'chr20', 'chrY', 'chr19', 'chr22', 'chr21', '@pool_1', '@pool_2'] : Threads : 4 : Sample: LNCaP_CelLin_BL_d0_BStg_R2_from_LNCaP-BL_diluted_0-065_41545.mapped.nodups Bam: /scratch/scratch/regmspi/results/totem/cellLine//LNCaP_CelLin_BL_d0_BStg_R2_from_LNCaP-BL_diluted_0-065_41545/LNCaP_CelLin_BL_d0_BStg_R2_from_LNCaP-BL_diluted_0-065_41545.mapped.nodups.bam : : Methylation Calling... 2020-11-06 20:57:12,735 ERROR: Process '/usr/local/lib/python3.8/dist-packages/gemBS/gemBSbinaries/bs_call' finished with 1 2020-11-06 20:57:12,736 ERROR: Additional threads: 2 1 1 2020-11-06 20:57:12,736 ERROR: Loading reference sequence index 2020-11-06 20:57:12,736 ERROR: Sequence index read in successfully 2020-11-06 20:57:12,736 ERROR: Opened /scratch/scratch/regmspi/results/totem/cellLine//LNCaP_CelLin_BL_d0_BStg_R2_from_LNCaP-BL_diluted_0-065_41545/LNCaP_CelLin_BL_d0_BStg_R2_from_LNCaP-BL_diluted_0-065_41545.mapped.nodups.bam for input (Index) 2020-11-06 20:57:12,736 ERROR: Processing region chr13:1-115169878 2020-11-06 20:57:12,736 ERROR: Processing chromosome chr13 (OK) 2020-11-06 20:57:12,736 ERROR: Loading reference for chr13 2020-11-06 20:57:12,736 ERROR: len = 115169878 2020-11-06 20:57:12,736 ERROR: Read in 96149878 bases on chr13 from 19020001 - 115169878, stored in 38459954 bytes 2020-11-06 20:57:12,736 ERROR: > System.Error::Signal raised (no=11) Exception in thread Thread-1: ValueError: Error while executing the bscall process. : Methylation call done, samples performed: LNCaP_CelLin_BL_d0_BStg_R2_from_LNCaP-BL_diluted_0-065_41545.mapped.nodups

Could you please help?

from gembs.

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