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traxexx avatar traxexx commented on August 19, 2024

idxdepth and "sd depth" are not the same thing.
"idxdepth" is the path to the output from running idxdepth, the depth estimation tool.
"depth sd" is the square root of "depth variance", used as a parameter in genotyping. If neither "depth variance" nor "depth sd" is specified, Paragraph will estimate that from your "depth" input.

I need to fix the error message and README.

from paragraph.

dantaki avatar dantaki commented on August 19, 2024

That's what I thought, and just to clarify I added those extra columns since I wanted Paragraph to know the sex of my samples since I am also using the X chromosome in genotyping. Therefore, I needed to put in those "depth sd" and "depth variance"

from paragraph.

traxexx avatar traxexx commented on August 19, 2024

Fix included in v2.4. now "depth sd" can be correctly recognized in manifest.
If you have "sex" column in the manifest, Paragraph will automatically adjust depth in chrX and chrY.

from paragraph.

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