Comments (6)
Have you solved this problem yet? I met this problem, too. Could you please share your experience for me?Thanks so much!
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No I haven't, but do let me know too if you have solved the problem - Thanks!
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I had solved it after i compressed .gft file to .gz format. I think there is a bug exist in "analyze.py", line 46 and line 47. I think you can also solve these problem through fixing this bug.
from alignqc.
Hi Szi Kay,
I think AlignQC works with gpd file not gtf. This could be a problem too.
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Sorry, my bad, just realised that gtf is actually preferred.
from alignqc.
Thank you @Yuyao1025, I agree it's the bug in line 46 and 47. The bug was fixed by removing the word "gzip" after open on line 47. So the following code works for anyone with the same issue:
if args.gtf[-3:] == '.gz': ginf = gzip.open(args.gtf)
else: ginf = open(args.gtf)
and thank you @abayega for commenting!
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Related Issues (20)
- ValueError HOT 14
- Adjust sort requirement to be compatible with any samtools sorted file HOT 1
- Isoforms
- Partial match/annotation HOT 6
- Error while reading reference fasta HOT 2
- Adapter sequences in reads
- Minimap2 compatibility HOT 3
- Error while reading index HOT 1
- ModuleNotFoundError: No module named 'analyze' HOT 4
- `bioconda` package HOT 3
- Error X11 module cannot be loaded
- input bam
- Short reads analysis HOT 1
- transcript read count from alignQC
- Memory Requirements HOT 4
- ValueError: Expected lines to be ordered but they appear not to be ordered on line 49447 HOT 1
- Hi @rojinsafavi Sorry for the delay. I'm a busy these days so if lose track of these I appreciate getting the reminder :) It looks like a problem streaming the data. Is your alignment file sorted by genomic position? If they are ... I have a second more complicated problem that this may be due to. If they are supported by position, do you know if the index of your chromosomes are in alphabetical order? I notice that different aligners have different behaviors when it comes to sorting and sometimes they sort chromosomes alphabetically ... sometimes they do other things. And i may be making the alphabetical assumption in the ordering-check. Something you can try is my sort tool thats in seqtools
- Expected lines to be ordered but they appear not to be ordered HOT 1
- problem with the installation via Conda HOT 1
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