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thanushipeiris avatar thanushipeiris commented on June 12, 2024

@jni Could you please provide a bit more explanation about how these two use cases differ? I am unfamiliar with them.

I think I understand what you mean by

treat the 2D image as a very thin 3D slice

That's like if you did a low res scan of a 3D volume and a high res imaging of an ROI in 2D then wanted to position your
ROI within the larger volume. But I don't get

treat it as "limited" to the two right-aligned dimensions

Is the neuron segmentation in 2D while the calcium imaging timeseries in 2D + t? So you'd just want to copy the same neuron segmentation over time?

from affinder.

jni avatar jni commented on June 12, 2024

Yep, both your interpretations are correct. The segmentation applies equally to every time point. One specific example that triggered this issue was aligning an island's contour coordinates (a shapes layer) with a time-series of surface temperature on the island.

from affinder.

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