Rscripts on sc-rnaseq, rnaseq, TPM normalization, and manuscript plots for the Bioinformatics project titled: Deconvolution of Cell Types from Bulk Gene Expression Profiling in a Non-alcoholic Steatohepatitis Model of Mice Liver Reveals Global Changes in Fibrosis and Immune Response Pathways
kiteomoru / deconvolution-analysis-of-mouse-liver-cell-types Goto Github PK
View Code? Open in Web Editor NEWThis project involves the Deconvolution (Digital flow-cytometry) of mice whole liver. ScRNA-Seq analysis was performed using Seurat R package, RNA-Seq pipeline was performed with HISAT2 (Alignment), HTSEQ (read count). Data normalization was performed, and the data was uploaded to CIBERSORTx to perform deconvolution analysis to identify the differences in cell type fractions between healthy samples and samples with Non-alcoholic Steatohepatitis (NASH).