Comments (7)
Config Error: Drivers::Muscle: Something went wrong with this alignment that was working on 6
sequences :/ You can find the output in this log file:
/tmp/tmp1jslp7u3/00_log.txt
What does the log file say, @Alesita0381?
from anvio.
@meren This is what the log file says:
DATE: 17 Mar 24 11:03:28
CMD LINE: muscle -quiet
THIS IS THE OUTPUT YOU ARE LOOKING FOR:
from anvio.
This is weird and makes no sense :(
Would you consider switching to the master branch and testing your run there, @Alesita0381?
from anvio.
from anvio.
@Alesita0381 , please install the development version of anvi'o and test the same command using that version :)
we cannot tell from the log file what is wrong with the muscle
run, but before we can do any debugging (which would happen in the development version anyway), we want to make sure that this remains a problem when you upgrade.
from anvio.
HI @meren I already installed what the instructions indicated and it worked, thank you!!!!!
I only have one question left. Why when I run anvi-interactive does it not show me the names of the bacteria in the phylogenetic tree? only appears the project name no the estimated taxonomy
i run like this:
anvi-interactive -p phylogenomic-profile.db -t phylogenomic-tree.txt --title "Pseudomonas_tree" --taxonomic-level t_species --manual
from anvio.
Hey @Alesita0381 you might get more prompt help/or perhaps find the answer on the Discord.
from anvio.
Related Issues (20)
- [BUG] pulp v2.8 made API changes that cause errors generating workflow DAG diagrams HOT 1
- [BUG] Can't create phylogenomic tree, as phylogenomic-tree.txt and default collection has different number of items.
- [BUG] Can't create phylogenomic tree, as phylogenomic-tree.txt and default collection has different number of items. HOT 3
- [BUG] Can't visualise the pangenome with average nucleotide identity HOT 1
- [BUG] anvi-setup-scgs-taxonomy STILL writes SCGs database to default location HOT 2
- [FEATURE REQUEST] add parameter --temp for temporary files path HOT 5
- [FEATURE REQUEST] Refactoring Anvio to be more eukaryote friendly/account for different genetic architectures
- [FEATURE REQUEST] Different gene caller option(s) for `anvi-gen-contigs-database` HOT 5
- [FEATURE REQUEST] See external/third-party dependencies on program documentation pages HOT 1
- [FEATURE REQUEST] Adding a program to annotate viral PHROGs via their HMMs
- [FEATURE REQUEST] A shortcut for importing layer orders that doesn't go through `anvi-import-misc-data`
- [FEATURE REQUEST] Environment variables for KEGG, Pfam, and other data directories so sys admins can set a global --xxxx-data-dir value
- [FEATURE REQUEST] Annotate which KOfams were added by our bitscore relaxation heuristic
- [FEATURE REQUEST] A conda package for anvi'o with a minimal installation option
- [BUG] `--prodigal-single-mode` breaks metagenomic workflow HOT 5
- [BUG] anvio-cluster-contigs fails with Generate input data
- [BUG] anvi-meta-pan-genome does not allow me to use gene calls not from prodigal HOT 10
- [BUG] Missing USearch in installation instruction and workflow DAG declarations HOT 1
- [BUG] contigs.db has issues when inputing aa_sequence in the external-gene-call file HOT 4
- [BUG] MaxBin2 failing due to one missing coverage HOT 2
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