Comments (2)
For this issue, i tried to find where the difference generated.
I believe that isoseq groupdedup
execution uses rc
tag in BAM files from isoseq correct
step.
I checked the cells having rc:i:1
which means predicted real cells in corrected.bam
file generating from isoseq correct
.
--> 10747
But bcstats.tsv from isoseq bcstats
with input file as corrected.bam
showed the real cell count --> 3225
The other sample: 8497 vs 8686
Does bcstats.tsv filter more when deciding the real cells?
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