linlin-coder
Have you had a chance to check out the docs? https://docs.qiime2.org There are many tutorials, walkthroughs, and guides available. If you still need help, please visit: https://forum.qiime2.org/c/user-support environment : qiime2 2023.05 - docker-image data:PACBIO sequel II my command as follows: ``` qiime tools import --type 'SampleData[SequencesWithQuality]' --input-path ../input-path-list.tsv --output-path ccs-data-demux.qza --input-format SingleEndFastqManifestPhred33V2 qiime cutadapt trim-single --i-demultiplexed-sequences ../ccs-data-demux.qza --p-cores 4 --p-adapter RGYTACCTTGTTACGACTT --p-front AGRGTTYGATYMTGGCTCAG --o-trimmed-sequences ccs-data-cutadapt.qza --output-dir result qiime deblur denoise-16S --i-demultiplexed-seqs ccs-data-cutadapt.qza --o-table deblur.table.qza --o-representative-sequences deblur.representative-sequences.qza --o-stats deblur.stat.qza --p-trim-length 10 --verbose ``` when i run those programm, something error information as follows: ``` File "/opt/conda/envs/qiime2-2023.5/lib/python3.8/site-packages/deblur/workflow.py", line 130, in trim_seqs for label, seq in input_seqs: File "/opt/conda/envs/qiime2-2023.5/lib/python3.8/site-packages/deblur/workflow.py", line 99, in sequence_generator for record in skbio.read(input_fp, format=format, **kw): File "/opt/conda/envs/qiime2-2023.5/lib/python3.8/site-packages/skbio/io/registry.py", line 506, in <genexpr> return (x for x in itertools.chain([next(gen)], gen)) File "/opt/conda/envs/qiime2-2023.5/lib/python3.8/site-packages/skbio/io/registry.py", line 531, in _read_gen yield from reader(file, **kwargs) File "/opt/conda/envs/qiime2-2023.5/lib/python3.8/site-packages/skbio/io/registry.py", line 1008, in wrapped_reader yield from reader_function(fhs[-1], **kwargs) File "/opt/conda/envs/qiime2-2023.5/lib/python3.8/site-packages/skbio/io/format/fastq.py", line 351, in _fastq_to_generator phred_scores, seq_header = _parse_quality_scores(fh, len(seq), File "/opt/conda/envs/qiime2-2023.5/lib/python3.8/site-packages/skbio/io/format/fastq.py", line 522, in _parse_quality_scores _decode_qual_to_phred(chunk, variant=variant, File "/opt/conda/envs/qiime2-2023.5/lib/python3.8/site-packages/skbio/io/format/_base.py", line 34, in _decode_qual_to_phred raise ValueError("Decoded Phred score is out of range [%d, %d]." ``` this error information is `ValueError: Decoded Phred score is out of range [0, 62]`,but data is correct when i import fastq data