filtering outputs from cutoff p-value=1 about jum

[qqwang-berkeley]

Yes you set the p-value cutoff to different values and JUM results will be filtered differently according to your setting. So you will see different results. A p-value at 1 is basically give you the full alternative splicing events in your sample (may or may not change significantly in your condition v.s. control)

[avantika-insitro]

Thank you. I understand that I can set the p-value threshold and filter the outputs of padj_1 to padj 0.05. What I do not understand right now is why the results of this filtering are different from setting 0.05 as the p-value cutoff to JUM. Shouldn't the results obtained by passing padj 0.05 be identical to those obtained by setting padj 1 and subsequently filtering to 0.05?

[qqwang-berkeley]

if you first run JUM with padj_1, you should be able to retrieve the exact same results by filtering the raw results using any new threshold values v.s. running JUM with padj of the desired value. Be careful about the filtering step, as it is tricky - you are filtering by the usage of a sub junction within an AS event, but need to report the AS event. Could you show me how exactly you filter JUM results? I can also send you a downstream script that I use to filter JUM results from padj 1