apolitis / eif3

Data and scripts to model eIF3 from MS data

eIF3

Written by Argyris Politis, email:[email protected]

Data and scripts to model eIF3 from MS data.

Steps

The method used here is the same as that used for the MS benchmark.

To generate models, run the ms_cg.py script. This will generate a number of models of the complex, named conf_eif3.<num>.pym.

The .pym files can be converted to .tcl files by running the pym2tcl.py script, and then to .mfj files by running the tcl2mfj.py script.

Info

Author(s): Argyris Politis

Version: 1.0

License: LGPL. This library is free software; you can redistribute it and/or modify it under the terms of the GNU Lesser General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version.

Last known good IMP version:

Testable: Yes.

Parallelizeable: No

Publications:

• Keren Lasker, Javier A. Velazquez-Muriel, Benjamin M. Webb, Zheng Yang, Thomas E. Ferrin, Andrej Sali, Macromolecular assembly structures by comparative modeling and electron microscopy, Methods in Molecular Biology, 2011.
• Politis A, Stengel F, Hall Z, Hernandez H, Leitner A, Waltzhoeni T, Robinson CV, Aebersold R. A mass spectrometry-based hybrid method for structural modelling of protein complexes. Nature Methods, 11, 403-406, (2014)