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pipeline.md

Trinity assembly

Assembly Vespertilio_sinensis

~/trinityrnaseq-v2.12.0/Trinity --seqType fq --left ../rawdata/QC/S7_1.clean.fq.gz,../rawdata/QC/S9_1.clean.fq.gz,../rawdata/QC/S11_1.clean.fq.gz,../rawdata/QC/S14_1.clean.fq.gz,../rawdata/QC/S15_1.clean.fq.gz,../rawdata/QC/S16_1.clean.fq.gz,../rawdata/QC/S18_1.clean.fq.gz,../rawdata/QC/S19_1.clean.fq.gz,../rawdata/QC/S20_1.clean.fq.gz,../rawdata/QC/S25_1.clean.fq.gz,../rawdata/QC/S26_1.clean.fq.gz,../rawdata/QC/S27_1.clean.fq.gz --right ../rawdata/QC/S7_2.clean.fq.gz,../rawdata/QC/S9_2.clean.fq.gz,../rawdata/QC/S11_2.clean.fq.gz,../rawdata/QC/S14_2.clean.fq.gz,../rawdata/QC/S15_2.clean.fq.gz,../rawdata/QC/S16_2.clean.fq.gz,../rawdata/QC/S18_2.clean.fq.gz,../rawdata/QC/S19_2.clean.fq.gz,../rawdata/QC/S20_2.clean.fq.gz,../rawdata/QC/S25_2.clean.fq.gz,../rawdata/QC/S26_2.clean.fq.gz,../rawdata/QC/S27_2.clean.fq.gz --CPU 30 --min_contig_length 150 --output Vespertilio_sinensis_trinity --max_memory 100G

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