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tseemann avatar tseemann commented on August 11, 2024

288681 is strain specific: Bacillus cereus E33L
86661 is more general Bacillus cereus group

So there was probably statistically enough E33L specific kmers for it to be binned as 33L.

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jenniferlu717 avatar jenniferlu717 commented on August 11, 2024

This has to do with Kraken's base algorithm. Kraken tries to give the most specific classification possible.

So for example, if two species A/B are in genus X and 2000 kmers classify at genus X but 30 kmers classify at species A and 2 kmers at species B, Kraken will give a classification of species A.

More generally, Kraken classifies reads at the leaf level of the root-to-leaf path with the most kmers.

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