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biospyder_app's Introduction

BioSpyder Sequence Analysis Tool

This is a fast tool for preliminary gene analysis which offers an interface for visualising descriptive metrics about the genes

This is the deployed version

This is the link to the repo

Installation and usage

In order to run the app locally, you will need to have conda installed.

Installation

Once the directory is been downloaded or cloned locally, you will need to create a conda environment: conda env create -f environment.yml

You can check for the successful creation of the environment using: conda spyder_app list

Once the environment is created, using the terminal:

  • Move into the directory cd biospyder_app
  • Activate the conda environment conda activate spyder_app
  • Run the app python app.py

The local host server will run on port 8000

Usage

Once the server is running, or the heroku server has responded, you will find yourself on the landing page, where you can upload a .fasta or .csv file containing genomic sequences. landing page

After uploading a file in the correct format, you will be prompted with the number of sequences loaded, as well as a dropdown menu indicating to select one of the sequences for further details. upload page

Once one of the sequences is chosen, the app will present you with metrics about the selected sequence, including length, base distribution and 10bp sequence with highest GC content. results page

Technologies

The app is based on Dash, but implements the use of pandas for data handling, pytest for testing and gunicorn for deploying.

Future features

Future features include:

  • Blasting the sequence against the NCBI database
  • Local alignment of sequences
  • Sequence translation and inference of 3D structure

biospyder_app's People

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