guyon-j / coculture_gliomas-neurons

Image process framework to easily analyse fluorescent glioblastoma cells in pattern of neurons.

An analysis tool for glioblastoma & neuron coculture

This tool proposes several worflow processes for analyzing cancer cells behaviors on a neuronal network. Have a look at the project's Cancer Feature Analysis to get more information about the image analysis tools.

Installation Instructions

In Fiji window, open Plugins > Macros > Startup Macros... and copy and past the code >> here <<. Change the language into IJ1 Macro and save as .ijm file in the Fiji.app folder > macros > toolsets.

Select the tool in the "More Tools" menu (>>)

Tool icons

1. Network Analysis
   The neuronal mat is converted into a simple network and tumor cells are added on the image. It provides information on intercellular contact and cellular morphology.
   
   

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2. Single Cell Tracking Analysis
   Using the shape of the cell migration, one cell can be selected and tracked.
   
   

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3. Multi Cell Tracking (preprocessing)
   Processing steps to binarize the stack and allows its analysis with already developped plugins.
   
   

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4. Spheroid Migration
   After delimitation of the pattern, the binarized area occupied by the spheroid is quantified. By relating the area of the spheroid to that of the pattern, we obtain a measure of the confluence.
   
   

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How to Cite

Guyon, J., Strale, P-O., Romero-Garmendia, I., Bikfalvi, A., Studer, V. and Daubon, T.

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions. DOI: 10.3791/62213.

Compatibility

This toolset was developed on window 10, and tested using ImageJ v1.53. Many features require installation of command line tools that may present future challenges on different operating systems.