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ambiguous base correction code write by Yusy
This project forked from virologist/ambiguous_base_v2
ambiguous base correction code write by Yusy
##################使用须知##################### 1.单条序列中歧义碱基的含量不应大于1%或2%,不推荐歧义碱基含量高的序列使用 2.校正歧义碱基的算法是根据汉明距离最近的序列,因此建议下载所有可得序列后进行去歧义碱基计算,最后再考虑去除重复或者相似度高的序列。 3.此脚本不合适校正遗传距离较远的序列歧义碱基位点 4.输入序列需经过比对和裁剪保留ORF,第一位密码子需位ATG三联体密码子。 5.在满足4的条件下,异常终止密码子为提前终止的三联体密码子,通常是由测序质量不佳,在拼接过程中出现的。 ###### Usage: python ambiguous_base.py input_align_sequences.fas Becareful, the input sequences (fas/fasta format) shoud be align and trim Function: correct the ambiguous base
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