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Platon can not get ORF id about platon HOT 4 CLOSED

oschwengers avatar oschwengers commented on May 27, 2024
Platon can not get ORF id

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Comments (4)

oschwengers avatar oschwengers commented on May 27, 2024

Hi @chen1i6c04 ,
thanks for the report. Could you please execute Platon in --verbose mode and provide me the log file produced by Platon and if possible the input fasta file as well?
Have you tried the bioconda version? If not, coud you please try this or provide me with the Prodigal-Version your using?

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chen1i6c04 avatar chen1i6c04 commented on May 27, 2024

Hi @oschwengers
My prodigal version is 2.6.3
and attach input fasta file, log file and prodigal gff file
hope they will help
platon.log
fasta.fa
prodigal.gff

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oschwengers avatar oschwengers commented on May 27, 2024

Hi @chen1i6c04 ,
I found the reason for this error. The fasta file you provided contains Windows ^M (carriage return) characters which are not common on linux systems on/for which platon has been developed. You simply have to remove these carriage returns and everything is back to normal.

On linux, you can do this via: sed $'s/\r//' R15.0599.txt > R15.0599.clean.txt

On this cleaned file you get the following results:

ID	Length	Coverage	# ORFs	RDS	Circular	Inc Type(s)	# Replication	# Mobilization	# OriT	# Conjugation	# AMRs	# rRNAs	# Plasmid Hits
contig54|Assembly_SPAdes|R15.0599	4872	NA	6	12.3	no	0	0	0	0	0	3	0	1
contig55|Assembly_SPAdes|R15.0599	4640	NA	5	14.4	no	1	0	0	0	0	3	0	1

Best reagards!

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chen1i6c04 avatar chen1i6c04 commented on May 27, 2024

It can work. Thanks very much.

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