when i want to run the demo ' python locnuclei.py example/ example/ result.out -t',
error happend, as fllows:
~/LocNuclei$ python locnuclei.py example/ example/ result.out -t
Namespace(blast_folder='example/', blast_suffix='.profile', debug=False, fasta_folder='example/', fasta_suffix='.fasta', only_blast=False, output_file='result.out', temp_folder=None, traveller=True, verbose=False)
Start: 2019-12-10 02:05:10.047608
b''
b'Invalid Symbol File Entry: Z\n'
ERROR: String-kernel returned with exit-code 1

#note:
because I just need use svm ,so I Commented two lines of code（don't use blast）：
def predict_given_files(self, fasta_folder, fasta_suffix, blast_folder, blast_suffix, tmp_folder, out_file, only_blast):

0) Read files and determine the workload

self.get_fasta_files(fasta_folder, fasta_suffix)
self.get_blast_files(blast_folder, blast_suffix)
self.prepare_temporary_directory(tmp_folder)

self.prepare_query_files(tmp_folder)

1) Ask Blast for homologues proteins - if we've a hit we don't need to run the whole SVM-process:

2 blaster = BlastPredictor(self.verbose, self.working_directory, self.file_manager, self.predict_traveller)

2 self.all_query_proteins = blaster.predict_all_query_proteins(self.all_query_proteins)

when i want to run the demo ' python locnuclei.py example/ example/ result.out -t',
error happend, as fllows:
~/LocNuclei$ python locnuclei.py example/ example/ result.out -t
Namespace(blast_folder='example/', blast_suffix='.profile', debug=False, fasta_folder='example/', fasta_suffix='.fasta', only_blast=False, output_file='result.out', temp_folder=None, traveller=True, verbose=False)
Start: 2019-12-10 02:05:10.047608
b''
b'Invalid Symbol File Entry: Z\n'
ERROR: String-kernel returned with exit-code 1

#note:
because I just need use svm ,so I Commented two lines of code（don't use blast）：
def predict_given_files(self, fasta_folder, fasta_suffix, blast_folder, blast_suffix, tmp_folder, out_file, only_blast):
# 0) Read files and determine the workload
self.get_fasta_files(fasta_folder, fasta_suffix)
self.get_blast_files(blast_folder, blast_suffix)
self.prepare_temporary_directory(tmp_folder)
# self.prepare_query_files(tmp_folder)
# 1) Ask Blast for homologues proteins - if we've a hit we don't need to run the whole SVM-process:
### 2 blaster = BlastPredictor(self.verbose, self.working_directory, self.file_manager, self.predict_traveller)
### 2 self.all_query_proteins = blaster.predict_all_query_proteins(self.all_query_proteins)

            # 2) Run SVMs for proteins without an blast-hit
            if only_blast == False:
                profkernel_predictor = SVMPredictor(self.verbose, self.working_directory, self.file_manager)
                self.all_query_proteins = profkernel_predictor.predict_all_query_proteins_without_blast_hit(self.all_query_proteins)

The file Amino.txt is referred to in external_file_manager.py as.
self._amino_file = '/usr/share/fastprofkernel/data/Amino.txt'

However, this file cannot be run and therefore the example cannot be executed.

Could you please provide this file..

When I ran the program, an error occurred .

D:\LocNuclei\LocNuclei>python locnuclei.py example/ example/ result.
Namespace(blast_folder='example/', blast_suffix='.profile', debug=False, fas
asta_suffix='.fasta', only_blast=False, output_file='result.out', temp_folde
verbose=False)
ERROR: File /usr/share/fastprofkernel/data/Amino.txt not available - exit!

however ,I can't find the file named Amino.txt