Neuroimmunology Scientist
Department of Neurology
University Hospital Düsseldorf
Heinrich Heine University Düsseldorf
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June 30, 2024: I will give an oral presentation about our recent work at The 40th Congress of the European Committee for Treatment and Research in Multiple Sclerosis, which will take place on 18-20 September 2024 in Copenhagen, Denmark. The presentation is titled "Secondary Autoimmunity after Alemtuzumab treatment in MS: Longitudinal immune repeertoire study suggests pre-existing predisposition ".
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May 10, 2024: I am going to present our recent work at Swiss Neurological Society (SNS) annual meeting 2024 , which will take place on June 6th, 2024 in Basel, Switzerland. The presentation is titled "Dynamic Reshaping of Lymphocyte Repertoires in Multiple Sclerosis Patients Treated with Alemtuzumab: Insights into Secondary Autoimmunity".
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July 02, 2024: Our recent article has been published in eBioMedicine: Christopher Nelke, Christina B. Schroeter, Sumanta Barman, Frauke Stascheit, Lars Masanneck, Lukas Theissen, Niklas Huntemann, Sara Walli, Derya Cengiz, Vera Dobelmann, Anna Vogelsang, Marc Pawlitzki, Saskia Räuber, Felix F. Konen, Thomas Skripuletz, Hans-Peter Hartung, Simone König, Andreas Roos, Andreas Meisel, Sven G. Meuth, and Tobias Ruck. The article is titled "Identification of disease phenotypes in acetylcholine receptor-antibody myasthenia gravis using proteomics-based consensus clustering" and for this study, I had the privilege of conducting the B cell receptor (BCR) repertoire analysis of myasthenia gravis patients using Python and R-based tools, a bash scripts-based pipeline, and custom scripts.
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June 10, 2024: Our recent article has been published in Nature Communications: Nicole Caduff, Lisa Rieble, Michelle Böni, Donal McHugh, Romin Roshan, Wendell Miley, Nazzarena Labo, Sumanta Barman, Matthew Trivett, Douwe M. T. Bosma, Julia Rühl, Norbert Goebels, Denise Whitby, Christian Münz. The article is titled "T cell specificity and protective functions elicited by KSHV infection of B cells in vivo" and I was involved in the TCR repertoire data analysis in EBV and EBV+KSHV infected humanized mice.
I am a neuroimmunology scientist with extensive expertise in central nervous system (CNS) autoimmune diseases. My research focuses on leveraging molecular biology, computational immunology, and cutting-edge '-omics' technologies to extract valuable insights and drive significant progress in the field of neuroimmunology. I possess extensive expertise in analysis of large multi-omics (single cell and multimodal transcriptomics, proteomics, single cell and bulk immunogenomics) data sets, next-generation sequencing (NGS), both in laboratory and computational, and single B cell recombinant antibody technology.
My mastery of data analysis using Python and R, coupled with machine learning and deep learning-based tools, empowers me to make informed decisions based on data and evidence. A significant aspect of my work involves data-driven investigation of treatment effects from various immunotherapies on patients' immune repertoires, as well as contributing to the development and characterization of recombinant human monoclonal antibodies derived from clonally expanded plasma cells (cePc). I possess good knowledge of statistical approaches, utilizing tools such as R, SPSS and GraphPad Prism, to derive meaningful insights and statistical interpretations in research. Furthermore, I am deeply passionate about the application of artificial intelligence (AI) in healthcare and its potential to revolutionize precision medicine.
My dedication and perseverance have resulted in numerous scientific publications and presentations, both oral and poster, at national and international conferences. Moreover, I have had the privilege of mentoring multicultural students in all aspects of research, including project conception, methodology, data analysis, presentation, and report writing for bachelor's, master's, and PhD theses.
- Molecular biology and computational immunology
- Multi-omics technologies
- Next generation sequencing (NGS)
- Bulk and single cell RNA-Seq data analysis
- Recombinant human monoclonal antibody technology
- NGS library preparation, data acquisition, quality control, visualization, statistical analysis, and interpretation
- Bulk RNA-seq, single cell RNA-Seq, CITE-seq, transcriptomics, proteomics, immunogenomics data analysis using R and Python-based tools, custom scripts and bash scripts-based pipeline
- Computational downstream analysis of scRNA-seq, immunogenomics data using machine and deep learning-based tools
- Mammalian cell culture
- Stable and transient cell line generation
- Cloning, Transformation, Transfection
- PCR, Single cell RT-PCR, qPCR
- Flow cytometry
- Affinity chromatography
- Cerebrospinalcord fluid (CSF) and peripheral blood (PB) single cell-based multi-omics approach (transcriptomics, proteomics, immune repertoire) on cohorts of different autoimmune encephalitis patients (anti-GAD, anti-IgLON5, Seronegative autoimmune encephalitis)
- CITE-seq data analysis of Batf+/+ and Batf-/- mouse bone marrow progenitor cells
- Investigate longitudinal treatment effects of anti-CD20 specific disease-modifying therapy on multiple sclerosis (MS) patients’ immune repertoire
- Investigate longitudinal treatment effects of anti-CD52 disease-modifying therapy on the peripheral immune repertoire of multiple sclerosis patients
- Investigate longitudinal treatment effects of S1P receptor modulator on autimmune lymphocyte repertoire in multiple sclerosis (MS)
- BCR repertoire data analysis in Myasthenia gravis (MG) patients
- TCR repertoire data analysis in EBV and EBV+KSHV infected humanized mice
- Development and characterization of recombinant human monoclonal antibodies from clonally expanded plasma cells (cePc) of patients with different autoimmune encephalitis (anti-GABA A, anti-IgLON5, anti-NMDAR, anti-CASPR2, anti-LGI1, anti-Hu)
- Recombinant monoclonal antibodies (anti-GABA A, NMDAR) effect on human brain organoid
- Harpes simples virus 1 (HSV1) encephalitis patient CSF derived recombinant human monoclonal antibody development and characterization
Programming and Scripting Languages: Python and R (advance), HTML (beginner)
Integrated Development Environments (IDEs) and Notebooks: RStudio, Jupyter Notebook, Visual Studio Code
Bioinformatics: Bioconductor, IMGT, IMGT/HighV-QUEST, BLAST, MAFFT, VDJdb etc.
Data Analysis and Visualization:
Single cell sequencing data analysis: Cell Ranger, STAR, Seurat, Scanpy, Scirpy, SCVItools, Monocle, Harmony, Dandelion, scRepertoire, NAIR, Propeller, Enclone, GLIPH, Immunearch, Immcantation, Dowser, Tcrdist3, Loupe Browser, Loupe V(D)J Browser, DESeq2, limma etc.Statistical Analysis: R, SPSS, GraphPad Prism
Bulk seq analysis: MiXCR, STAR, VDJtools, Immunearch, Immcantation
Others: R Markdown, Pandas, NumPy, Ggplot2, Plotly, Matplotlib, UMAP, t-SNE, TCRex, ViClod etc.
Operating system: Linux, Mac-OS, Windows
Containerization: Docker
Version Control: Git
Others: High-performance computing, Machine learning and Deep learning (Intermediate)
Computational immunology:
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Alemtuzumab-induced immune phenotype and repertoire changes: implications for secondary autoimmunity
Authors:Tobias Ruck#, Sumanta Barman#, Andreas Schulte-Mecklenbeck#, Steffen Pfeuffer#, Falk Steffen, Christopher Nelke, Christina B Schroeter, Alice Willison, Michael Heming, Thomas Müntefering, Nico Melzer, Julia Krämer, Maren Lindner, Marianne Riepenhausen, Catharina C Gross, Luisa Klotz, Stefan Bittner, Paolo A Muraro, Tilman Schneider-Hohendorf, Nicholas Schwab, Gerd Meyer zu Hörste, Norbert Goebels, Sven G Meuth, Heinz Wiendl. #equally contributing first authorsJournal: Brain
Year: 2022
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Identification of disease phenotypes in acetylcholine receptor-antibody myasthenia gravis using proteomics-based consensus clustering
Authors:Christopher Nelke, Christina B. Schroeter, Sumanta Barman, Frauke Stascheit, Lars Masanneck, Lukas Theissen, Niklas Huntemann, Sara Walli, Derya Cengiz, Vera Dobelmann, Anna Vogelsang, Marc Pawlitzki, Saskia Räuber, Felix F. Konen, Thomas Skripuletz, Hans-Peter Hartung, Simone König, Andreas Roos, Andreas Meisel, Sven G. Meuth, and Tobias Ruck.Journal: eBioMedicine
Year: 2024
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Classifying flow cytometry data using Bayesian analysis helps to distinguish ALS patients from healthy controls
Authors:Saskia Räuber, Christopher Nelke, Christina B Schroeter, Sumanta Barman, Marc Pawlitzki, Jens Ingwersen, Katja Akgün, Rene Günther, Alejandra P Garza, Michaela Marggraf, Ildiko Rita Dunay, Stefanie Schreiber, Stefan Vielhaber, Tjalf Ziemssen, Nico Melzer, Tobias Ruck, Sven G Meuth, Michael Herty.Journal: Frontiers in Immunology
Year: 2023
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T cell specificity and protective functions elicited by KSHV infection of B cells in vivo
Authors:Nicole Caduff, Lisa Rieble, Michelle Böni, Donal McHugh, Romin Roshan, Wendell Miley, Nazzarena Labo, Sumanta Barman, Matthew Trivett, Douwe M. T. Bosma, Julia Rühl, Norbert Goebels, Denise Whitby, Christian Münz.Journal: Nature Communications
Year: 2024
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Dynamic reshaping of lymphocyte repertoires in multiple sclerosis patients treated with Alemtuzumab: Insights into secondary autoimmunity
Authors: Sumanta Barman et al.
Ready to submit -
Comprehensive compartment-specific single-cell multi-omics profiling in GAD-autoimmunity
Authors: Sumanta Barman, Saskia Räuber et al.
On preparation
Neuroimmunology:
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NMDAR encephalitis: passive transfer from man to mouse by a recombinant antibody
Authors:Manish Malviya#, Sumanta Barman#, Kristin S Golombeck#, Jesús Planagumà#, Francesco Mannara#, Nathalie Strutz‐Seebohm, Claudia Wrzos, Fatih Demir, Christine Baksmeier, Julia Steckel, Kim Kristin Falk, Catharina C Gross, Stjepana Kovac, Kathrin Bönte, Andreas Johnen, Klaus‐Peter Wandinger, Elena Martín‐García, Albert J Becker, Christian E Elger, Nikolaj Klöcker, Heinz Wiendl, Sven G Meuth, Hans‐Peter Hartung, Guiscard Seebohm, Frank Leypoldt, Rafael Maldonado, Christine Stadelmann, Josep Dalmau, Nico Melzer, Norbert Goebels. #equally contributing first authorsJournal: Annals of Clinical and Translational Neurology
Year: 2017
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Myelinating Co-Culture as a Model to Study Anti-NMDAR Neurotoxicity
Authors: Mercedeh Farhat Sabet, Sumanta Barman, Mathias Beller, Sven G Meuth, Nico Melzer, Orhan Aktas, Norbert Goebels, Tim Prozorovski.
Journal: International Journal of Molecular Sciences
Year: 2023
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An assay to determine mechanisms of rapid autoantibody-induced neurotransmitter receptor endocytosis and vesicular trafficking in autoimmune encephalitis
Authors: Elsie Amedonu, Christoph Brenker, Sumanta Barman, Julian A Schreiber, Sebastian Becker, Stefan Peischard, Nathalie Strutz-Seebohm, Christine Strippel, Andre Dik, Hans-Peter Hartung, Thomas Budde, Heinz Wiendl, Timo Strünker, Bernhard Wünsch, Norbert Goebels, Sven G Meuth, Guiscard Seebohm, Nico Melzer
Journal: Frontiers in Neurology
Year: 2019
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Dose‐dependent inhibition of demyelination and microglia activation by IVIG
Authors: Meike Winter, Christine Baksmeier, Julia Steckel, Sumanta Barman, Manish Malviya, Melanie Harrer‐Kuster, Hans‐Peter Hartung, Norbert Goebels
Journal: Annals of Clinical and Translational Neurology
Year: 2016
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N‐methyl‐D‐aspartate receptor antibodies in autoimmune encephalopathy alter oligodendrocyte function
Authors: Carlos Matute, Ana Palma, María Paz Serrano‐Regal, Estibaliz Maudes, Sumanta Barman, María Victoria Sánchez‐Gómez, María Domercq, Norbert Goebels, Josep Dalmau.
Journal: Annals of Neurology
Year: 2020
Selected conference publications:
- Postdoc Workshop - Drug Hunting @ Bayer, Heiligenhaus, Germany, September 21-24, 2022
- American Academy of Neurology Annual Meeting, Seattle, USA, April 2-7, 2022
- ECTRIMS, Stockholm, Sweden, September 11-13, 2019
- Novartis Research Day 2019, Berlin, Germany, January 24-25, 2019
- 14th International Congress of Neuroimmunology, Brisbane, Australia, August 27-31, 2018
- You can also find a complete list of my publications here.
- Heine Research Academies (HeRA) travel grant for participating in the 2022 American Academy of Neurology annual meeting, Seattle, USA
- Best poster prize award, Novartis Research Day MS 2019, Berlin, Germany
- International Society of Neuroimmunology travel grant for participating in the 14th International Congress of Neuroimmunology, Brisbane, Australia
- Received prize for academic excellence in Bachelor of Science
- Received scholarships for academic excellence in grade 5 and grade 8
- Playing musical instruments
- Photography
- Hiking
- Camping
- Cycling
- Coding
- Writing
Coming soon....
Feel free to reach out if you are interested in collaboration or have any inquiries related to my research!
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