Comments (2)
Managed to resolve following the subsequent steps:
- Loaded bzip2 using
module load bzip2
- Learned
pip install pysam
fails withpython==3.8
- downgradedpython==3.7
- Got pysam installation error:
creating build/temp.linux-x86_64-cpython-37/pysam
gcc -pthread -B /nfs/home/vbezshapkin/miniconda3/envs/mvirs/compiler_compat -Wl,--sysroot=/ -Wsign-compare -DNDEBUG -g -fwrapv -O3 -Wall -Wstrict-prototypes -fPIC -I/tmp/pip-install-o58dw52c/pysam_e5a03d4c9d1a4ad18f23c1fb1e922126/htslib -I/tmp/pip-install-o58dw52c/pysam_e5a03d4c9d1a4ad18f23c1fb1e922126/samtools -I/tmp/pip-install-o58dw52c/pysam_e5a03d4c9d1a4ad18f23c1fb1e922126/samtools/lz4 -I/tmp/pip-install-o58dw52c/pysam_e5a03d4c9d1a4ad18f23c1fb1e922126/bcftools -I/tmp/pip-install-o58dw52c/pysam_e5a03d4c9d1a4ad18f23c1fb1e922126/pysam -I/tmp/pip-install-o58dw52c/pysam_e5a03d4c9d1a4ad18f23c1fb1e922126 -I/nfs/home/vbezshapkin/miniconda3/envs/mvirs/include/python3.7m -c htslib/bcf_sr_sort.c -o build/temp.linux-x86_64-cpython-37/htslib/bcf_sr_sort.o -Wno-unused -Wno-strict-prototypes -Wno-sign-compare -Wno-error=declaration-after-statement
as: unrecognized option '--gdwarf-5'
- Installed
pysam
using conda - Run
pip install mvirs
from mvirs.
The final de novo solution is:
$ conda create -n mvirs python==3.7 pip bwa samtools pysam -c bioconda
$ conda activate mvirs
$ python -m pip install mvirs
from mvirs.
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from mvirs.