tiffkwin / kfw-backend

A backend Flask application for kfwx ⚙️🧬

kfw-backend

This is a toolkit for MacOS X and Windows that automatically analyzes data for assays.

Understanding the Output

This section explains in detail the significance of the results of the data analysis.

Membrane Potential

1. Metadata Sheet - This sheet hosts information about the experiment itself
   • ID - The experimental ID.
   • Standard Curve - A true or false value indicating whether or not the standard curve calculation was used.
   • Slope - The slope value entered by the researcher to be used in calculations.
   • Y-Intercept - The y-intercept value entered by the researcher to be used in calculations.
   • Substrates - A column containing the list of substrates used in the experiment.
   • Additions - A column containing the list of additions used in the experiment.
   • Date - The date the analysis was run in the form YYYY-MM-DD.
2. Raw Data - The raw data taken from the lab tools.
   • The data is in the form X | Y | X |...
3. Averaged Raw Data - The average membrane potential for 3-minute (i.e., 180 seconds) increments in each data set. Each data set is annotated with the buffer conditions of the assay at that time.
4. Corrected Raw Data - The same data as the Average Raw Data sheet, but with an additional correction calculation which divides each average membrane potential by the the average membrane potential for Ala.
5. Standard Curve Data - The results of the standard curve calculation on the raw data.
   • The data is in the form X | Y | X |...
6. Averaged Standard Curve - The results of the standard curve calculation on the averaged data.

NADH Redox

1. Metadata Sheet - This sheet hosts information about the experiment itself.
   • ID - The experimental ID.
   • Substrates - A column containing the list of substrates used in the experiment.
   • Additions - A column containing the list of additions used in the experiment.
   • Date - The date the analysis was run in the form YYYY-MM-DD.
2. Raw Data - The raw data taken from the lab tools.
   • The data is in the form X | Y | X |...
3. Stripped Data - Contains only the columns that correspond to the NADH data points.
   • The data is in the form X | Y | X |...
4. Reduced Data - The same data as the Stripped Data sheet, but with an additional reduction calculation.
   • y_new = (y_old-min)/(max-min)*100
5. Averaged Reduced Data - The averages of the reduced data for 3-minute (i.e., 180 seconds) increments in each data set. Each data set is annotated with the buffer conditions of the assay at that time.

H2O2

1. Metadata Sheet - This sheet hosts information about the experiment itself
   • ID - The experimental ID.
   • Standard Curve - A true or false value indicating whether or not the standard curve calculation was used.
   • Slope - The slope value entered by the researcher to be used in calculations.
   • Y-Intercept - The y-intercept value entered by the researcher to be used in calculations.
   • Substrates - A column containing the list of substrates used in the experiment.
   • Additions - A column containing the list of additions used in the experiment.
   • Mitochondria - The amount of mitochondria use (in milligrams).
   • Date - The date the analysis was run in the form YYYY-MM-DD.
2. Raw Data - The raw data taken from the lab tools.
   • The data is in the form X | Y | X |...
3. Slopes Data - The slope for 3-minute (i.e., 180 seconds) increments in each data set. Each data set is annotated with the buffer conditions of the assay at that time.
4. Corrected Slopes - The same data as the Slopes sheet, but with an additional correction calculation which divides each slope value by the mg of mitochondria used.
5. Standard Curve - The results of the standard curve calculation on the Raw Data.
6. Standard Curve Slopes - The results of the standard curve calculation on the Slopes Data.
7. Corrected Standard Curve Slopes - The results of the correction calculation on the Standard Curve Slopes data.

Membrane Potential Merge

Combines data from all of the Averaged Standard Curve sheets in the Membrane Potential data analysis Excel files. Each row corresponds to a substrate. The Mean and SEM is calculated for each row.

NADH Redox Merge

Combines data from all of the Averaged Reduced Data sheets in the NADH Redox data analysis Excel files. Each row corresponds to a substrate. The Mean and SEM is calculated for each row.

H2O2 Merge

Combines data from all of the Corrected Standard Curve Slopes sheets in the H2O2 data analysis Excel files. Each row corresponds to a substrate. The Mean and SEM is calculated for each row.

kfw-backend's Issues

🧵⏳Implement session tracking

Need a way to track user sessions

• Cookies?
• Use session to create a user ID

📑🤔 Fix file storage so it can handle multiple documents

Right now DB committing can only work with one file

• fix dat

💾 Set up the database

• Download db viewer
• Set up sqlalchemy
• Set up sqlite