- BugBase installed and able to run test dataset
- This git repository
- Graphlan installed to generate Figure 4
HMP OTU table was generated using QIIME v.1.8.0 (closed reference) with the GreenGenes 97% representative set v.13.5.8 and was filtered to keep only samples with at least 500 counts and samples from the tongue dorsum, stool and plaque
Human Microbiome Project Consortium. Structure, function and diversity of the healthy human microbiome. Nature 486, 207–214 (2012).
- HMP_Map.txt
- HMP_OTUS.biom
The OTU table for the western, non-western stool analysis was pulled from the Qitta websiteand was filtered to keep samples from only non-infants (>3 years of age) and samples with a minimum of 500 sequence counts
Yatsunenko, T. et al. Human gut microbiome viewed across age and geography. Nature 486, 222–227 (2012).
- Yats_Adult_Map.txt
- Yats_Adult_OTUs.biom
The OTU table for the Yellowstone hotspring analysis was pulled from the Qitta website and was filtered to keep only water samples and samples with a minimum of 500 sequence counts
This data is not published elsewhere (yet!)
- Yellow_Stone_Map.txt
- Yellow_Stone_OTUs.biom
The OTU table for the soil analysis was pulled from the Qitta website and was filtered to keep samples with a minimum of 500 sequence counts
Bartram, A. K. et al. Exploring links between pH and bacterial community composition in soils from the Craibstone Experimental Farm. FEMS Microbiol. Ecol. 87, 403–415 (2014).
- Soil_Map.txt
- Soil_OTUs.biom
The vagina OTU table was generated using NINJA-OPS (closed reference) with the GreenGenes 97% representative set v13.5.8 all samples had a minimum of 300 sequences
Ravel, J. et al. Vaginal microbiome of reproductive-age women. Proc. Natl. Acad. Sci. 108, 4680–4687 (2011).
- Vagina_Map.txt
- Vagina_OTUs.biom
To complete the BugBase analysis for all the work presented in the publication, you can run the commands listed in the BugBase_Workflow.txt
file.
These commands should be run from within the BugBase_Manuscript main directory.