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Course Syllabus - Special Topics in Evolutionary Biology:

Macroevolution & Phylogenetics

BIOL 6014 TR 11:00 - 12:15 PM

Professor: Prof. Josef Uyeda (Yo-sef Weh-duh) Email: [email protected]

All lectures will conducted in person

Course location: Goodwin Hall 241

Class website: Canvas and https://github.com/uyedaj/macrophy_course

Discussion site: vt-macrophy-2022.slack.com

Office hours: Thursday at 9:00am EST or by appointment using Calendly.

Textbooks: None are required, readings will be provided from the primary literature, as well as from the following texts. Purchase of these texts is optional, though both are great reference material for those interested in phylogenetics and phylogenetic methods.

Baum, David A. and Stacey D. Smith. Tree Thinking: An Introduction to Phylogenetic Biology (1st Edition). W.H. Freeman, 2012.

Felsenstein, Joseph. Inferring Phylogenies (2nd Edition). Sinauer, 2003.

Harmon, Luke. Phylogenetic Comparative Methods: Learning from trees. CC-BY-4.0,2018.

**Course Description: **

Phylogenetic trees are the map by which we understand evolutionary history and ultimately, all of biology. The goals of this course are threefold:

  1. Embrace tree-thinking: You will learn what a phylogeny represents, how to use them to interpret evolutionary history and importantly, how to estimate them from biological data. This is the study of phylogenetics.

  2. Unleash the power of the comparative method: Why should we estimate trees in the first place? We will use phylogenies as a map for studying macroevolutionary questions about trait evolution, the relationships between organisms and their abiotic and biotic environment, and the causes and consequences of diversification. This is the study of phylogenetic comparative methods.

  3. Think big - Macroevolutionary science: We will engage with the rich and exciting history of evolutionary thought which fundamentally sought to unite microevolutionary processes, genetics, and development to explain macroevolutionary patterns across the tree of life. We will read and discuss about these “big ideas” in macroevolution, and discuss how recent advances in phylogenetics and comparative methods enable us to test these ideas in ways never before possible.

Course Policies

Our Inclusive Classroom It is my goal to foster an inclusive classroom environment in both face-to-face and virtual settings. This means that students from all diverse backgrounds and perspectives are entitled to a safe, welcoming and respectful environment free from prejudice, bullying, discrimination and bias - including in the form of microaggressions, posting material insensitive or insulting to others, or any form of online harassment. By enrolling and attending this course, you agree to be respectful of diversity across gender identity, sexuality, disability, age, socioeconomic status, ethnicity, race, nationality, and culture. We acknowledge and open to honest dialogue regarding the history of racism within evolutionary biology and the role it has played, and continues to play, in our macroevolution and phylogenetics research communities. This course will effort to acknowledge and work to remedy that harm. Your feedback, suggestions and comments are always appreciated and welcome.

University Honor Code “As a Hokie, I will conduct myself with honor and integrity at all times. I will not lie, cheat, or steal, nor will I accept the actions of those who do.” Students enrolled in this course are responsible for abiding by the Honor Code. A student who has doubts about how the Honor Code applies to any assignment is responsible for obtaining specific guidance from the course instructor before submitting the assignment for evaluation. Ignorance of the rules does not exclude any member of the University community from the requirements and expectations of the Honor Code. Academic integrity expectations are the same for online classes as they are for in person classes. All university policies and procedures apply in any Virginia Tech academic environment. For additional information about the Honor Code, please visit: https://www.honorsystem.vt.edu/

Attendance Please make an effort to attend as many lectures as you can. Materials will be posted to both Canvas and the github site, except for materials with student information (e.g. class recordings), which will only be posted on Canvas. You are expected to participate in all discussions, have completed assigned readings BEFORE class, and to fully engage with the material and your classmates. Students missing several class periods should consult with the instructor and can attend office hours to get back up to speed.

Course Syllabus The course syllabus is subject to change by the instructor. Changes will be announced in class and on Canvas. Exam dates are unlikely to be changed from their original version.

Student Evaluation Exams (2 X 100 pts, 200pts; at least one of these exams will be take-home) Participation in discussions of primary literature and in-class activities (50 pts) Exercises (~ 5 assignments, each worth 10 pts, 50 pts) Final Project: Final project presentation(100 pts)

Final Project. All course participants will conduct a final research project that will conduct an analysis of phylogenetic data using the methods and techniques learned in class. This project is largely open-ended and up to the student. However, suggested topics include: building a phylogeny of a group of taxa of interest using available sequence data on Genbank, analyzing existing phenotypic datasets and phylogenies with phylogenetic comparative methods, or conducting a simulation study of phylogenetic model behavior. One of the 5 assignments is to submit a project proposal (due 9/29) that outlines the research question under study, the proposed datasets to be used, the analyses to be conducted, and the expected results (No more than 2 pages, not including citations). The last 2-3 class periods will be spent for final presentations in the form of 15 minute talks on the results of these independent research projects. We will not meet for the final exam time.

Technology. All material will be posted on Canvas and github, except for recorded lectures/discussion, which will only be posted on Canvas. The provided Slack channel is available for students to connect to their instructor and each other to work on projects, develop ideas, discuss topics, complete assignments and otherwise build our virtual community. As stated above, the code of conduct and University principles of community extend to this virtual setting.

Services for Students with Disabilities (SSD) Virginia Tech welcomes students with disabilities into the University’s educational programs. The University promotes efforts to provide equal access and a culture of inclusion without altering the essential elements of coursework. If you anticipate or experience academic barriers that may be due to disability, including but not limited to, chronic medical conditions, Deaf or hard of hearing, learning disability, mental health, or vision impairment, please contact the Services for Students with Disabilities (SSD) (540-231-3788, [email protected], or visit www.ssd.vt.edu). If you have an SSD accommodation letter, please meet with me privately during office hours as early in the semester as possible to discuss implementing your accommodations. You must give me reasonable notice to implement your accommodations, which is generally 5 business days and 10 business days for final exams.

Course Schedule

Date Topic Reading Assignments
T 8/23 1. An Introduction to Phylogenetics & Macroevolution, pdf
Th 8/25 2. Parsimony & cladistics;pdf Baum&Smith Ch 1-3
T 8/30 3. Felsenstein & the birth of statistical phylogenetics; pdf Baum&Smith Ch 4 & 7 Assignment I: Self-evaluation
Th 9/1 4. Probability, likelihood & Rev. Bayes; Answers Felsenstein 1981
T 9/6 5. Discrete character evolution I;pdf O’Meara 2012
Th 9/8 6. Discrete character evolution II;pdf
T 9/13 7. Slides;pdf Lab: Inferring phylogenies from molecular data Inferring phylogenies Chs. 13, 16
Th 9/15 8. PAUP* Lab
T 9/20 9. EXAM I
Th 9/22 10. Practical considerations: alignments, support, model selection & adequacy; pdf Holder & Lewis, 2003; Brown, 2014 PAUP lab due (log file)
T 9/27 11. Revbayes Lab
T 9/29 12. Model selection & adequacy; pdf. Assignment: Project proposal
Th 10/4 13. Phylogenomics.
T 10/6 14. Dating phylogenetic trees; pdf Heath et al. 2014; Gavryushkina et al. 2017; Landis et al. 2018
Th 10/11 15. Species trees & the multispecies coalescent; pdf Edwards 2009; Optional review: 10 years later, Bravo et al. 2019
T 10/13 16. Brownian Motion & continuous trait evolution;pdf Lab
Th 10/18 17. The comparative method & PICs;pdf Felsenstein 1985
T 10/20 18. Modeling adaptation;pdf Hansen 1997
Th 10/25 19. Ornstein-Uhlenbeck models II;pdf Butler and King 2004
T 10/27 19. [Postdoc Guest Lecturer(s) TBD]
Th 11/1 20. Rethinking comparative methods;pdf Uyeda et al. 2018
T 11/3 23. Hidden State Models Beaulieu et al. 2013
Th 11/8 24. EXAM II
T 11/10 25. Diversification models - birth and death on trees; pdf Harmon Textbook Ch. 10-12
Th 11/15 26. Species selection - SSE models; pdf Maddison et al. 2007
T 11/17 27. Punctuated Equilibrium; pdf Eldredge & Gould 1972; Charlesworth et al. 1982
Th 11/22 Thanksgiving Holiday
T 11/24 Thanksgiving Holiday
Th 11/29 28.Grand Challenges/Connecting Micro & Macroevolution;pdf Hansen and Houle 2004
M 12/1 29. Presentations
Th 12/6 30. Presentations

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