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Chl_Fluorescent

本程序用于分析叶绿素荧光数据,原始数据为使用Agilent Cary Eclipse 荧光分光光度计及自制配件生成。

使用方法:

-1:将需分析的.csv文件(文件名不能含有中文)和 Chlorophyll_fluorescence.exe 放置到同一文件夹。该文件夹内不能含有其他.csv文件。

-2:检查每个.csv文件的数据格式,具体要求包括 (1)第一行为文件名或样品名。(2)第二行为标题Times 和Intensity。 (3)以下各行分别为时间和强度的数值。 (4)除此之外不含有其他数据。

-3:双击Chlorophyll_fluorescence.exe运行程序,将进行第一个.csv文件的可视化,具体包括两张图。 第一张为原始信号和降噪后信号展示,可用于判断降噪效果及可信度。 第二张为可交互的降噪后信号图,鼠标左键点击数据点,可显示横纵坐标具体数值,一般来说需确定F0,Fm,F0‘,Fm’的具体数值,这些数值需手动记录。 如需保存图片,可点击保存按钮保存。

-4:记录所需参数后,可关闭两张图,此时将自动展示第二个.csv文件结果。以此类推,直到分析完所有.csv文件。

V0.1: 2023/4/16, Damo版本开发,实现小波降噪并展示数据的基本功能。

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