xueliang4906 / polysome_detect

MATLAB scripts to analyze polysomes formed by ribosomes based on their coordinates and rotations.

Polysome_detect

MATLAB script to annotate polysomes in cellular tomograms based on ribosome coordinate and rotation information from subtomo analysis/averaging.

This script was first described in the manuscript by Xue et al, 2021. Preprint on bioRxiv.

Basic concepts

1. A motivelist(motl) table to combine and store all relevant information for each ribosomes, such as coordinates, rotations, class info, etc.

2. Use ribosomes' mRNA exit to entry distance to define polysomes and their sequence.

3. Extract quantitative information on polysome arrangement, such as overall distribution, relative orientations, etc.

How to run

Require MATLAB 2016b or equivalent.

Put the two associated fromEuler_RELION.m and rotmat2eulang.m files in MATLAB search Path.

Open the polysome_detect.m, and run it step by step (select and F9).