Comments (1)
Hi @suhuanhou,
Thank you for your interest in our method! Bisque expects a counts matrix and internally performs CPM normalization. While applying CPM normalization on top of TPM normalization gives you the same TPM values, the results in the manuscript are based off of CPM normalization alone. Bisque's behavior with TPM values has not been tested and we suggest adhering to the raw counts input.
Best,
Brandon
from bisque.
Related Issues (20)
- Adipose dataset HOT 1
- how to prepare single cell input from multiple subjects as an input? HOT 1
- sim.data average proportions taking into account variation between replicates HOT 1
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from bisque.