Comments (1)
Thank you -- I had come across this before but had no chance to implement a solution yet. The issue seems to be that the reverse_complement()
API got changed in Biopython 1.80. There seems to be a bit of inconsistency between the various Seq class types and inconsistency between Seq and SeqRecord's reverse_complement()
function. I suspect that in DNA Cauldron the class inheritance causes problem somehow.
Here is the PR of the Biopython change: biopython/biopython#3648
A suggested solution is biopython/biopython#4177 (comment) but not sure that will be so easy.
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Related Issues (15)
- Check parts assembling into enzyme sites
- pdf_reports required but not installed by pip HOT 1
- The tutorial uses parts as keyword argument for SequenceRepository but now its collections as dict of dict HOT 1
- Annotating features with qualifiers HOT 1
- Purpose of OligoPairAnnealing class (oligo_annealing method in assembly plan) HOT 2
- Assembling fragments digested by different enzymes HOT 5
- Test fails with Biopython v1.79 HOT 1
- example with ladders HOT 1
- problem with snapgene_reader HOT 3
- Unwanted case-sensitivity when indexing fragment against source record HOT 2
- Palindromes in Golden Gate assembly, only want one construct HOT 5
- Linear assemblies? HOT 3
- annotations crossing overhangs lost HOT 7
- Choosing enzyme in AssemblyPlan.from_spreadsheet()
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