gear-genomics / indigo Goto Github PK

Hello!
Thank you for a great tool!

Could you help me, please, to install and run this package locally? Are there any instructions for this?

Hello There,

What other parameters are used, beside the peak ratio, in 'Indigo' web application? Because I seeing a bit different results compared to my Tracy run.

Thank you.

Hello,

This is clearly an excellent tool that you have developed for Sanger analysis. My only suggestion so far would be if the colors of the tracings on the pdf file could be more intense and closer to the "standard" dNTPs, please see attached file.

Cheers
Ghalib

3788.pdf

Hello,

I am trying to call variants from a human gene using ab1 files with indigo web server.
In the result page every time I've downloaded the bcf file it has errors. The result page seems normal, I can copy and paste the variants in separate file, but the downloaded bcf file does not works.
This is what it looks like my bcf file.

Thanks,

Isabela

1. I downloaded the samples.abi file
2. I uploaded it and used both GRCh37 and GRCh38 and clicked analysis
3. I get the error Error in running Indigo: Couldn't anchor the Sanger trace in the selected reference genome.