QC CRISPR Pooled Libraries in Addgene
CRISPR Pooled Libraries on Addgene
- library type: activation, knockout and inhibition
- species: human and mouse
- only genome-wide libraries showed here
1. activation + human (5 libraries)
Name |
ID |
Library Type |
Species |
PI |
Lentiviral Generation |
gRNAs per gene |
Total gRNAs |
Broad GPP activation Calabrese p65-HSF |
92379 (Set A), 92380 (Set B) |
Activation |
Human |
Doench and Root |
3rd |
3–6 |
56,762 (Set A), 56,476 (Set B) |
CRISPRa-v2 |
83978, 1000000091 |
Activation |
Human |
Weissman |
3rd |
5, 10 |
104,540/209,080 |
Human CRISPR lncRNA Activation Pooled Library |
1000000106 |
Activation |
Human |
Zhang |
3rd |
10 |
96,458 |
SAM v1 - 3 plasmid system |
1000000057 (Zeocin) / 1000000074 (Puromycin) |
Activation |
Human |
Zhang |
3rd |
3 |
70,290 |
SAM v2 - 2 plasmid system |
1000000078 (Blasticidin) |
Activation |
Human |
Zhang |
3rd |
3 |
70,290 |
2. activate + mouse (3 libraries)
Name |
ID |
Library Type |
Species |
PI |
Lentiviral Generation |
gRNAs per gene |
Total gRNAs |
Broad GPP activation Calabrese p65-HSF |
92383 (Set A), 92384 (Set B) |
Activation |
Mouse |
Doench and Root |
3rd |
3–6 |
67,187 (Set A) / 66,889 (Set B) |
CRISPRa-v2 |
83996, 1000000093 |
Activation |
Mouse |
Weissman |
3rd |
5, 10 |
107,105 / 214,210 |
SAM v1 - 3 plasmid system |
1000000075 (Puromycin) |
Activation |
Mouse |
Zhang |
3rd |
3 |
69,716 |
3. knockout + human (9 libraries)
Name |
ID |
Library Type |
Species |
PI |
Lentiviral Generation |
gRNAs per gene |
Total gRNAs |
Activity-optimized genome-wide library |
1000000100 Knockout Human Sabatini and Lander 3rd 10 187,535 |
|
|
|
|
|
|
Broad GPP genome-wide Brunello |
73179 (1 plasmid) / 73178 (2 plasmid) |
Knockout |
Human |
Doench and Root |
3rd |
4 |
76,441 |
Human GeCKO v2 |
1000000048 (1 plasmid), 1000000049 (2 plasmid) |
Knockout |
Human |
Zhang |
3rd |
6 |
123,411 |
Human genome-wide library v1 |
69763 |
Knockout |
Human |
Wu |
3rd |
4 |
77,406 |
Human improved genome-wide library v1 |
67989 Knockout Human Yusa |
3rd |
5 |
90,709 |
|
|
|
Mini-human AsCpf1-based Human Genome-wide Knockout Library |
130630 |
Knockout |
Human |
Draetta |
3rd |
3-4 |
17,032 arrays |
Toronto KnockOut - Version 1 |
1000000069 |
Knockout |
Human |
Moffat |
3rd |
12 |
176,500 |
Toronto KnockOut - Version 3 |
90294 (1 plasmid), 125517 (2 plasmid) |
Knockout |
Human |
Moffat |
3rd |
4 |
70,948 |
Two plasmid human activity-optimized genome-wide library |
1000000095 |
Knockout |
Human |
Sabatini and Lander |
3rd |
10 |
187,536 |
4. knockout + mouse (5 libraries)
5. inhibition + human (2 libraries)
Name |
ID |
Library Type |
Species |
PI |
Lentiviral Generation |
gRNAs per gene |
Total gRNAs |
Broad GPP inhibition Dolcetto |
92385 (Set A) / 92386 (Set B) |
Inhibition |
Human |
Doench and Root |
3rd |
3–6 |
57,050 (Set A) / 57,011 (Set B) |
CRISPRi-v2 |
83969, 1000000090 |
Inhibition |
Human |
Weissman |
3rd |
5, 10 |
104,535 / 209,070 |
6. inhibition + mouse (2 libraries)
Name |
ID |
Library Type |
Species |
PI |
Lentiviral Generation |
gRNAs per gene |
Total gRNAs |
Broad GPP inhibition Dolomiti |
104090 (Set A) / 104091 (Set B) |
Inhibition |
Mouse |
Doench and Root |
3rd |
3, 6 |
67,366 (Set A) / 67,194 (Set B) |
CRISPRi-v2 |
83987, 1000000092 |
Inhibition |
Mouse |
Weissman |
3rd |
5, 10 |
107,415 /214,830 |