File Not Found Error during assembly_report execution about dnaweaver

[Zulko]

Note that while the error says "file not found", it could be the BLAST program itself that is actually not found. Did you install BLAST on your computer and so do you have a blast command accessible from the terminal?

[igemmadrid2021]

@Zulko Thank you for your quick answer!

Yes, I had ncbi blast+ installed and also pyblast3 and biopython. Actually after removing all the installations and re-installing again I manage to fix the issue with the unfound files. However, now it seems that the os.remove line in the sequence alignemtn script (blast_sequence) is having some trouble with that.

`File "C:\ProgramData\Anaconda3\libsite-packages\dnaweaver\biotools[sequence_homologies.py](http://sequence_homologies.py/)", line 121, in blast_sequence
os.remove(xml_name)

PermissionError: [WinError 32] The process does not have access to the file because it is being used by another process: 'C:\Users\fmjor\AppData\Local\Temp\tmpcg0sdkys.xml`

I am starting getting used with the inter-talk between python and windows, but I am really looking forward to learn in the process and implement DNAWeaver as well as other scripts from the BioFoundry within my lab workflows.

Thanks in advance for all the help!

EDIT: I have tried to run the same code in Linux and it goes super smooth. It may be an issue with the os module and windows.