Comments (3)
If I understand correctly, for PartsLibrary to support the circular sequences, the sequence decomposer has to work differently, or the sequence has to be re-indexed so that it started with insert. In this case, the sequence decomposer will find it.
from dnaweaver.
It is difficult to say for sure without a minimal runnable example but I believe you are right in your assumptions:
- DnaWeaver expects backbones to be represented like other parts: the linear sequence of the backbone that will end up in the final constructs, flanked by two restriction sites. If you loaded the complete backbone (as a circular construct) the parts library won't know what to do with it.
- DnaWeaver won't deal correctly with parts and backbones if they are appear cut in the
final
sequence you submit (e.g. one bit of the backbone of the left and another bit on the right).
Does that make sense?
from dnaweaver.
Yeah, it does! I will try to play with it. Thanks, @Zulko!
from dnaweaver.
Related Issues (12)
- dealing with circular DNA HOT 18
- Example of using PartsLibrary with Gibson assembly method HOT 2
- File Not Found Error during assembly_report execution HOT 2
- What to do when Gibson assembly Station is refused : Apologies to put a routine use question as an issue HOT 10
- Contact Zulko HOT 7
- verify_constraints performance HOT 3
- SBOL => Automation MoClo Assembly tool => Optimal assembly report HOT 2
- What role cut_spread_radius plays in circular sequence decomposition? HOT 1
- Q: Any specific reason why PcrExtractionStation not using the PartsLibrary? HOT 1
- Bug: Max length initialisation HOT 2
- Bug: When quote.assembly_plan is None
Recommend Projects
-
React
A declarative, efficient, and flexible JavaScript library for building user interfaces.
-
Vue.js
🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.
-
Typescript
TypeScript is a superset of JavaScript that compiles to clean JavaScript output.
-
TensorFlow
An Open Source Machine Learning Framework for Everyone
-
Django
The Web framework for perfectionists with deadlines.
-
Laravel
A PHP framework for web artisans
-
D3
Bring data to life with SVG, Canvas and HTML. 📊📈🎉
-
Recommend Topics
-
javascript
JavaScript (JS) is a lightweight interpreted programming language with first-class functions.
-
web
Some thing interesting about web. New door for the world.
-
server
A server is a program made to process requests and deliver data to clients.
-
Machine learning
Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.
-
Visualization
Some thing interesting about visualization, use data art
-
Game
Some thing interesting about game, make everyone happy.
Recommend Org
-
Facebook
We are working to build community through open source technology. NB: members must have two-factor auth.
-
Microsoft
Open source projects and samples from Microsoft.
-
Google
Google ❤️ Open Source for everyone.
-
Alibaba
Alibaba Open Source for everyone
-
D3
Data-Driven Documents codes.
-
Tencent
China tencent open source team.
from dnaweaver.