Does the PartsLibrary work for circular sequences? about dnaweaver

[andrewshvv]

If I understand correctly, for PartsLibrary to support the circular sequences, the sequence decomposer has to work differently, or the sequence has to be re-indexed so that it started with insert. In this case, the sequence decomposer will find it.

[Zulko]

It is difficult to say for sure without a minimal runnable example but I believe you are right in your assumptions:

• DnaWeaver expects backbones to be represented like other parts: the linear sequence of the backbone that will end up in the final constructs, flanked by two restriction sites. If you loaded the complete backbone (as a circular construct) the parts library won't know what to do with it.
• DnaWeaver won't deal correctly with parts and backbones if they are appear cut in the final sequence you submit (e.g. one bit of the backbone of the left and another bit on the right).

Does that make sense?

[andrewshvv]

Yeah, it does! I will try to play with it. Thanks, @Zulko!