enormandeau / ncbi_blast_tutorial Goto Github PK

Hi I am getting this error when trying to use the makeblastdb?

makeblastdb -in /lustre/project/taw/kvigil/Reference/viral.1.protein.faa.gz -dbtype prot

Building a new DB, current time: 10/27/2023 10:27:07
New DB name: /lustre/project/taw/kvigil/Reference/viral.1.protein.faa.gz
New DB title: /lustre/project/taw/kvigil/Reference/viral.1.protein.faa.gz
Sequence type: Protein
Keep MBits: T
Maximum file size: 1000000000B
BLAST options error: /lustre/project/taw/kvigil/Reference/viral.1.protein.faa.gz does not match input format type, default input type is FASTA

I run makeblastdb -in test3.fasta -title reference -dbtype nucl -out Data_test3 but got an error at the end as below:

Building a new DB, current time: 11/04/2023 09:06:17
New DB name: /Users/xiaolei/Downloads/Peng Zhang/BPHunter_fastq2BP_test/Data_test3
New DB title: reference
Sequence type: Nucleotide
Deleted existing Nucleotide BLAST database named /Users/xiaolei/Downloads/Peng Zhang/BPHunter_fastq2BP_test/Data_test3
Keep MBits: T
Maximum file size: 3000000000B
Adding sequences from FASTA; added 1 sequences in 0.000292778 seconds.

BLAST Database error: No alias or index file found for nucleotide database [/Users/xiaolei/Downloads/Peng] in search path [/Users/xiaolei/Downloads/Peng Zhang/BPHunter_fastq2BP_test::]

Is there a way to fix this issue?

Thanks,
Xiao

Sorry, a mistake.

Hello,

I am trying to install 2.9.0 version on my ubuntu but still appears the old version installed 2.6.0. How Can I remove the old version to work with the new one?

Thank you!