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splaisan avatar splaisan commented on July 23, 2024 1

I will first thing monday and post here. I will also rebuild a conda using the new conda version which now finally uses mamba_lib to search for packages (was about time they switched). This only has fixed another issue I had this week.
Good WE!

from bakta.

splaisan avatar splaisan commented on July 23, 2024 1

Hi @oschwengers,

I tried it once more with my existing env to confirm it fails (it did)
I deleted my conda and created a new one with conda 23.
It now works through 'predict & annotate CDSs...' and end with all expected results

It confirms that the new conda 23.10.0 with mamba resole did build a good environment

problem solved for me
thanks for your support

I reproduce here the last failed run and list of packages as well as the new list of packages in case that can help you figure out who was guilty. I strongly suspect pyrodigal 2.3 now instead of 3.10 before !

conda previous bad env

bakta --db /data/biodata/bakta_db/db --genus "${genus}"   --species "${species}"   --strain "${strain}"   --prefix "bc${bc}"   --complete   --output out bc01_assembly.fasta

parse genome sequences...
        imported: 3
        filtered & revised: 3
        chromosomes: 1
        plasmids: 2

start annotation...
predict tRNAs...
        found: 74
predict tmRNAs...
        found: 1
predict rRNAs...
        found: 18
predict ncRNAs...
        found: 34
predict ncRNA regions...
        found: 7
predict CRISPR arrays...
        found: 2
predict & annotate CDSs...
Traceback (most recent call last):
  File "/opt/miniconda3/envs/bakta/bin/bakta", line 10, in <module>
    sys.exit(main())
  File "/opt/miniconda3/envs/bakta/lib/python3.10/site-packages/bakta/main.py", line 228, in main
    cdss = feat_cds.predict(genome, contigs_path)
  File "/opt/miniconda3/envs/bakta/lib/python3.10/site-packages/bakta/features/cds.py", line 41, in predict
    orffinder = pyrodigal.OrfFinder(meta=prodigal_metamode, closed=closed)
AttributeError: module 'pyrodigal' has no attribute 'OrfFinder'
(bakta) $ conda list
# packages in environment at /opt/miniconda3/envs/bakta:
#
# Name                    Version                   Build  Channel
_libgcc_mutex             0.1                 conda_forge    conda-forge
_openmp_mutex             4.5                       2_gnu    conda-forge
about-time                4.2.1              pyhd8ed1ab_0    conda-forge
alive-progress            3.0.1              pyhd8ed1ab_0    conda-forge
aragorn                   1.2.41               h031d066_1    bioconda
archspec                  0.2.1              pyhd8ed1ab_1    conda-forge
bakta                     1.8.2              pyhdfd78af_0    bioconda
biopython                 1.81            py310h2372a71_1    conda-forge
blast                     2.14.0               h7d5a4b4_1    bioconda
brotli-python             1.1.0           py310hc6cd4ac_1    conda-forge
bzip2                     1.0.8                h7f98852_4    conda-forge
c-ares                    1.20.1               hd590300_1    conda-forge
ca-certificates           2023.7.22            hbcca054_0    conda-forge
certifi                   2023.7.22          pyhd8ed1ab_0    conda-forge
cffi                      1.16.0          py310h2fee648_0    conda-forge
charset-normalizer        3.3.1              pyhd8ed1ab_0    conda-forge
circos                    0.69.9               hdfd78af_0    bioconda
curl                      8.4.0                hca28451_0    conda-forge
diamond                   2.1.8                h43eeafb_0    bioconda
entrez-direct             16.2                 he881be0_1    bioconda
expat                     2.5.0                hcb278e6_1    conda-forge
font-ttf-dejavu-sans-mono 2.37                 hab24e00_0    conda-forge
font-ttf-inconsolata      3.000                h77eed37_0    conda-forge
font-ttf-source-code-pro  2.038                h77eed37_0    conda-forge
font-ttf-ubuntu           0.83                 hab24e00_0    conda-forge
fontconfig                2.14.2               h14ed4e7_0    conda-forge
fonts-conda-ecosystem     1                             0    conda-forge
fonts-conda-forge         1                             0    conda-forge
freetype                  2.12.1               h267a509_2    conda-forge
gettext                   0.21.1               h27087fc_0    conda-forge
giflib                    5.2.1                h0b41bf4_3    conda-forge
grapheme                  0.6.0              pyhd8ed1ab_0    conda-forge
hmmer                     3.3.2                hdbdd923_4    bioconda
icu                       73.2                 h59595ed_0    conda-forge
idna                      3.4                pyhd8ed1ab_0    conda-forge
infernal                  1.1.4           pl5321h031d066_4    bioconda
isa-l                     2.30.0               hd590300_6    conda-forge
keyutils                  1.6.1                h166bdaf_0    conda-forge
krb5                      1.21.2               h659d440_0    conda-forge
ld_impl_linux-64          2.40                 h41732ed_0    conda-forge
lerc                      4.0.0                h27087fc_0    conda-forge
libblas                   3.9.0           19_linux64_openblas    conda-forge
libcblas                  3.9.0           19_linux64_openblas    conda-forge
libcurl                   8.4.0                hca28451_0    conda-forge
libdeflate                1.19                 hd590300_0    conda-forge
libedit                   3.1.20191231         he28a2e2_2    conda-forge
libev                     4.33                 h516909a_1    conda-forge
libexpat                  2.5.0                hcb278e6_1    conda-forge
libffi                    3.4.2                h7f98852_5    conda-forge
libgcc-ng                 13.2.0               h807b86a_2    conda-forge
libgd                     2.3.3                h119a65a_9    conda-forge
libgfortran-ng            13.2.0               h69a702a_2    conda-forge
libgfortran5              13.2.0               ha4646dd_2    conda-forge
libgomp                   13.2.0               h807b86a_2    conda-forge
libidn2                   2.3.4                h166bdaf_0    conda-forge
libjpeg-turbo             3.0.0                hd590300_1    conda-forge
liblapack                 3.9.0           19_linux64_openblas    conda-forge
libnghttp2                1.55.1               h47da74e_0    conda-forge
libnsl                    2.0.1                hd590300_0    conda-forge
libopenblas               0.3.24          pthreads_h413a1c8_0    conda-forge
libpng                    1.6.39               h753d276_0    conda-forge
libsqlite                 3.43.2               h2797004_0    conda-forge
libssh2                   1.11.0               h0841786_0    conda-forge
libstdcxx-ng              13.2.0               h7e041cc_2    conda-forge
libtiff                   4.6.0                ha9c0a0a_2    conda-forge
libunistring              0.9.10               h7f98852_0    conda-forge
libuuid                   2.38.1               h0b41bf4_0    conda-forge
libwebp                   1.3.2                h658648e_1    conda-forge
libwebp-base              1.3.2                hd590300_0    conda-forge
libzlib                   1.2.13               hd590300_5    conda-forge
ncbi-amrfinderplus        3.11.26              h283d18e_0    bioconda
ncurses                   6.4                  h59595ed_1    conda-forge
numpy                     1.26.0          py310hb13e2d6_0    conda-forge
openssl                   3.1.4                hd590300_0    conda-forge
pbzip2                    1.1.13               h1fcc475_2    conda-forge
pcre                      8.45                 h9c3ff4c_0    conda-forge
perl                      5.32.1          4_hd590300_perl5    conda-forge
perl-archive-tar          2.40            pl5321hdfd78af_0    bioconda
perl-b-cow                0.007           pl5321h166bdaf_0    conda-forge
perl-carp                 1.50            pl5321hd8ed1ab_0    conda-forge
perl-clone                0.46            pl5321h166bdaf_0    conda-forge
perl-common-sense         3.75            pl5321hd8ed1ab_0    conda-forge
perl-compress-raw-bzip2   2.201           pl5321h166bdaf_0    conda-forge
perl-compress-raw-zlib    2.202           pl5321h166bdaf_0    conda-forge
perl-config-general       2.65            pl5321hdfd78af_0    bioconda
perl-digest-perl-md5      1.9             pl5321hdfd78af_2    bioconda
perl-encode               3.19            pl5321h166bdaf_0    conda-forge
perl-exporter             5.74            pl5321hd8ed1ab_0    conda-forge
perl-exporter-tiny        1.002002        pl5321hd8ed1ab_0    conda-forge
perl-extutils-config      0.008           pl5321ha770c72_0    conda-forge
perl-extutils-helpers     0.026           pl5321ha770c72_0    conda-forge
perl-extutils-installpaths 0.012           pl5321ha770c72_0    conda-forge
perl-extutils-makemaker   7.70            pl5321hd8ed1ab_0    conda-forge
perl-extutils-pl2bat      0.005           pl5321ha770c72_0    conda-forge
perl-font-ttf             1.06            pl5321hdfd78af_1    bioconda
perl-gd                   2.76            pl5321h5b5514e_1    bioconda
perl-inc-latest           0.500           pl5321ha770c72_0    conda-forge
perl-io-compress          2.201           pl5321hdbdd923_2    bioconda
perl-io-string            1.08            pl5321hdfd78af_4    bioconda
perl-io-zlib              1.14            pl5321hdfd78af_0    bioconda
perl-json                 4.10            pl5321hdfd78af_0    bioconda
perl-json-xs              2.34            pl5321h4ac6f70_6    bioconda
perl-list-moreutils       0.430           pl5321hdfd78af_0    bioconda
perl-list-moreutils-xs    0.430           pl5321h031d066_2    bioconda
perl-math-bezier          0.01            pl5321hdfd78af_2    bioconda
perl-math-round           0.07            pl5321ha770c72_0    conda-forge
perl-math-vecstat         0.08            pl5321hdfd78af_2    bioconda
perl-module-build         0.4232          pl5321ha770c72_0    conda-forge
perl-module-build-tiny    0.039           pl5321h166bdaf_0    conda-forge
perl-module-implementation 0.09            pl5321ha770c72_0    conda-forge
perl-module-runtime       0.016           pl5321ha770c72_0    conda-forge
perl-number-format        1.76            pl5321hdfd78af_0    bioconda
perl-params-validate      1.31            pl5321hec16e2b_0    bioconda
perl-parent               0.241           pl5321hd8ed1ab_0    conda-forge
perl-pathtools            3.75            pl5321h166bdaf_0    conda-forge
perl-readonly             2.05            pl5321ha770c72_0    conda-forge
perl-regexp-common        2017060201      pl5321hd8ed1ab_0    conda-forge
perl-scalar-list-utils    1.63            pl5321h166bdaf_0    conda-forge
perl-set-intspan          1.19            pl5321hdfd78af_2    bioconda
perl-statistics-basic     1.6611          pl5321hdfd78af_3    bioconda
perl-storable             3.15            pl5321h166bdaf_0    conda-forge
perl-svg                  2.87            pl5321hd8ed1ab_0    conda-forge
perl-test-fatal           0.016           pl5321ha770c72_0    conda-forge
perl-text-format          0.62            pl5321hdfd78af_0    bioconda
perl-time-hires           1.9764          pl5321h031d066_4    bioconda
perl-try-tiny             0.31            pl5321ha770c72_0    conda-forge
perl-types-serialiser     1.01            pl5321hdfd78af_0    bioconda
perl-xml-parser           2.44_01         pl5321hc3e0081_1003    conda-forge
pigz                      2.8                  h2797004_0    conda-forge
piler-cr                  1.06                 h4ac6f70_4    bioconda
pip                       23.3.1             pyhd8ed1ab_0    conda-forge
psutil                    5.9.5           py310h2372a71_1    conda-forge
pycparser                 2.21               pyhd8ed1ab_0    conda-forge
pyhmmer                   0.10.4          py310h4b81fae_0    bioconda
pyrodigal                 3.1.0           py310h4b81fae_0    bioconda
pysocks                   1.7.1              pyha2e5f31_6    conda-forge
python                    3.10.13         hd12c33a_0_cpython    conda-forge
python-isal               1.5.1           py310h2372a71_0    conda-forge
python_abi                3.10                    4_cp310    conda-forge
pyyaml                    6.0.1           py310h2372a71_1    conda-forge
readline                  8.2                  h8228510_1    conda-forge
requests                  2.31.0             pyhd8ed1ab_0    conda-forge
setuptools                68.2.2             pyhd8ed1ab_0    conda-forge
tk                        8.6.13               h2797004_0    conda-forge
trnascan-se               2.0.12          pl5321h031d066_0    bioconda
tzdata                    2023c                h71feb2d_0    conda-forge
urllib3                   2.0.7              pyhd8ed1ab_0    conda-forge
wget                      1.20.3               ha35d2d1_1    conda-forge
wheel                     0.41.3             pyhd8ed1ab_0    conda-forge
xopen                     1.8.0           py310hff52083_0    conda-forge
xz                        5.2.6                h166bdaf_0    conda-forge
yaml                      0.2.5                h7f98852_2    conda-forge
zlib                      1.2.13               hd590300_5    conda-forge
zstandard                 0.21.0          py310h1275a96_1    conda-forge
zstd                      1.5.5                hfc55251_0    conda-forge

conda 23.0.1 new working env

# packages in environment at /opt/miniconda3/envs/bakta:
#
# Name                    Version                   Build  Channel
_libgcc_mutex             0.1                 conda_forge    conda-forge
_openmp_mutex             4.5                       2_gnu    conda-forge
about-time                4.2.1              pyhd8ed1ab_0    conda-forge
alive-progress            3.0.1              pyhd8ed1ab_0    conda-forge
aragorn                   1.2.41               h031d066_1    bioconda
archspec                  0.2.2              pyhd8ed1ab_0    conda-forge
bakta                     1.8.2              pyhdfd78af_1    bioconda
biopython                 1.81            py310h2372a71_1    conda-forge
blast                     2.15.0          pl5321h6f7f691_1    bioconda
brotli-python             1.1.0           py310hc6cd4ac_1    conda-forge
bzip2                     1.0.8                hd590300_5    conda-forge
c-ares                    1.21.0               hd590300_0    conda-forge
ca-certificates           2023.7.22            hbcca054_0    conda-forge
certifi                   2023.7.22          pyhd8ed1ab_0    conda-forge
cffi                      1.16.0          py310h2fee648_0    conda-forge
charset-normalizer        3.3.2              pyhd8ed1ab_0    conda-forge
circos                    0.69.9               hdfd78af_0    bioconda
curl                      8.4.0                hca28451_0    conda-forge
diamond                   2.1.8                h43eeafb_0    bioconda
entrez-direct             16.2                 he881be0_1    bioconda
expat                     2.5.0                hcb278e6_1    conda-forge
font-ttf-dejavu-sans-mono 2.37                 hab24e00_0    conda-forge
font-ttf-inconsolata      3.000                h77eed37_0    conda-forge
font-ttf-source-code-pro  2.038                h77eed37_0    conda-forge
font-ttf-ubuntu           0.83                 hab24e00_0    conda-forge
fontconfig                2.14.2               h14ed4e7_0    conda-forge
fonts-conda-ecosystem     1                             0    conda-forge
fonts-conda-forge         1                             0    conda-forge
freetype                  2.12.1               h267a509_2    conda-forge
gettext                   0.21.1               h27087fc_0    conda-forge
giflib                    5.2.1                h0b41bf4_3    conda-forge
grapheme                  0.6.0              pyhd8ed1ab_0    conda-forge
hmmer                     3.3.2                hdbdd923_4    bioconda
icu                       70.1                 h27087fc_0    conda-forge
idna                      3.4                pyhd8ed1ab_0    conda-forge
infernal                  1.1.4           pl5321h031d066_4    bioconda
isa-l                     2.30.0               hd590300_6    conda-forge
jpeg                      9e                   h0b41bf4_3    conda-forge
keyutils                  1.6.1                h166bdaf_0    conda-forge
krb5                      1.21.2               h659d440_0    conda-forge
ld_impl_linux-64          2.40                 h41732ed_0    conda-forge
lerc                      4.0.0                h27087fc_0    conda-forge
libblas                   3.9.0           19_linux64_openblas    conda-forge
libcblas                  3.9.0           19_linux64_openblas    conda-forge
libcurl                   8.4.0                hca28451_0    conda-forge
libdeflate                1.17                 h0b41bf4_0    conda-forge
libedit                   3.1.20191231         he28a2e2_2    conda-forge
libev                     4.33                 h516909a_1    conda-forge
libexpat                  2.5.0                hcb278e6_1    conda-forge
libffi                    3.4.2                h7f98852_5    conda-forge
libgcc-ng                 13.2.0               h807b86a_3    conda-forge
libgd                     2.3.3                h5aea950_4    conda-forge
libgfortran-ng            13.2.0               h69a702a_3    conda-forge
libgfortran5              13.2.0               ha4646dd_3    conda-forge
libgomp                   13.2.0               h807b86a_3    conda-forge
libiconv                  1.17                 h166bdaf_0    conda-forge
libidn2                   2.3.4                h166bdaf_0    conda-forge
liblapack                 3.9.0           19_linux64_openblas    conda-forge
libnghttp2                1.58.0               h47da74e_0    conda-forge
libnsl                    2.0.1                hd590300_0    conda-forge
libopenblas               0.3.24          pthreads_h413a1c8_0    conda-forge
libpng                    1.6.39               h753d276_0    conda-forge
libsqlite                 3.44.0               h2797004_0    conda-forge
libssh2                   1.11.0               h0841786_0    conda-forge
libstdcxx-ng              13.2.0               h7e041cc_3    conda-forge
libtiff                   4.5.0                h6adf6a1_2    conda-forge
libunistring              0.9.10               h7f98852_0    conda-forge
libuuid                   2.38.1               h0b41bf4_0    conda-forge
libwebp                   1.2.4                h1daa5a0_1    conda-forge
libwebp-base              1.2.4                h166bdaf_0    conda-forge
libxml2                   2.9.14               h22db469_4    conda-forge
libzlib                   1.2.13               hd590300_5    conda-forge
ncbi-amrfinderplus        3.11.26              h283d18e_0    bioconda
ncbi-vdb                  3.0.8                hdbdd923_0    bioconda
ncurses                   6.4                  h59595ed_2    conda-forge
numpy                     1.26.0          py310hb13e2d6_0    conda-forge
openssl                   3.1.4                hd590300_0    conda-forge
ossuuid                   1.6.2             hf484d3e_1000    conda-forge
pbzip2                    1.1.13               h1fcc475_2    conda-forge
pcre                      8.45                 h9c3ff4c_0    conda-forge
perl                      5.32.1          4_hd590300_perl5    conda-forge
perl-alien-build          2.48            pl5321hec16e2b_0    bioconda
perl-alien-libxml2        0.17            pl5321hec16e2b_0    bioconda
perl-archive-tar          2.40            pl5321hdfd78af_0    bioconda
perl-b-cow                0.007           pl5321h166bdaf_0    conda-forge
perl-business-isbn        3.007           pl5321hd8ed1ab_0    conda-forge
perl-business-isbn-data   20210112.006    pl5321hd8ed1ab_0    conda-forge
perl-capture-tiny         0.48            pl5321ha770c72_1    conda-forge
perl-carp                 1.50            pl5321hd8ed1ab_0    conda-forge
perl-clone                0.46            pl5321h166bdaf_0    conda-forge
perl-common-sense         3.75            pl5321hd8ed1ab_0    conda-forge
perl-compress-raw-bzip2   2.201           pl5321h166bdaf_0    conda-forge
perl-compress-raw-zlib    2.202           pl5321h166bdaf_0    conda-forge
perl-config-general       2.65            pl5321hdfd78af_0    bioconda
perl-constant             1.33            pl5321hd8ed1ab_0    conda-forge
perl-digest-perl-md5      1.9             pl5321hdfd78af_2    bioconda
perl-encode               3.19            pl5321h166bdaf_0    conda-forge
perl-exporter             5.74            pl5321hd8ed1ab_0    conda-forge
perl-exporter-tiny        1.002002        pl5321hd8ed1ab_0    conda-forge
perl-extutils-config      0.008           pl5321ha770c72_0    conda-forge
perl-extutils-helpers     0.026           pl5321ha770c72_0    conda-forge
perl-extutils-installpaths 0.012           pl5321ha770c72_0    conda-forge
perl-extutils-makemaker   7.70            pl5321hd8ed1ab_0    conda-forge
perl-extutils-pl2bat      0.005           pl5321ha770c72_0    conda-forge
perl-ffi-checklib         0.28            pl5321hdfd78af_0    bioconda
perl-file-chdir           0.1011          pl5321hd8ed1ab_0    conda-forge
perl-file-path            2.18            pl5321hd8ed1ab_0    conda-forge
perl-file-temp            0.2304          pl5321hd8ed1ab_0    conda-forge
perl-file-which           1.24            pl5321hd8ed1ab_0    conda-forge
perl-font-ttf             1.06            pl5321hdfd78af_1    bioconda
perl-gd                   2.76            pl5321h5b5514e_1    bioconda
perl-importer             0.026           pl5321hd8ed1ab_0    conda-forge
perl-inc-latest           0.500           pl5321ha770c72_0    conda-forge
perl-io-compress          2.201           pl5321hdbdd923_2    bioconda
perl-io-string            1.08            pl5321hdfd78af_4    bioconda
perl-io-zlib              1.14            pl5321hdfd78af_0    bioconda
perl-json                 4.10            pl5321hdfd78af_0    bioconda
perl-json-xs              2.34            pl5321h4ac6f70_6    bioconda
perl-list-moreutils       0.430           pl5321hdfd78af_0    bioconda
perl-list-moreutils-xs    0.430           pl5321h031d066_2    bioconda
perl-math-bezier          0.01            pl5321hdfd78af_2    bioconda
perl-math-round           0.07            pl5321ha770c72_0    conda-forge
perl-math-vecstat         0.08            pl5321hdfd78af_2    bioconda
perl-module-build         0.4234          pl5321ha770c72_0    conda-forge
perl-module-build-tiny    0.039           pl5321h166bdaf_0    conda-forge
perl-module-implementation 0.09            pl5321ha770c72_0    conda-forge
perl-module-runtime       0.016           pl5321hdfd78af_2    bioconda
perl-number-format        1.76            pl5321hdfd78af_0    bioconda
perl-params-validate      1.31            pl5321h031d066_2    bioconda
perl-parent               0.241           pl5321hd8ed1ab_0    conda-forge
perl-path-tiny            0.124           pl5321hd8ed1ab_0    conda-forge
perl-pathtools            3.75            pl5321h166bdaf_0    conda-forge
perl-readonly             2.05            pl5321ha770c72_0    conda-forge
perl-regexp-common        2017060201      pl5321hd8ed1ab_0    conda-forge
perl-scalar-list-utils    1.63            pl5321h166bdaf_0    conda-forge
perl-scope-guard          0.21            pl5321hd8ed1ab_0    conda-forge
perl-set-intspan          1.19            pl5321hdfd78af_2    bioconda
perl-statistics-basic     1.6611          pl5321hdfd78af_3    bioconda
perl-storable             3.15            pl5321h166bdaf_0    conda-forge
perl-sub-info             0.002           pl5321hd8ed1ab_0    conda-forge
perl-svg                  2.87            pl5321hd8ed1ab_0    conda-forge
perl-term-table           0.016           pl5321hdfd78af_0    bioconda
perl-test-fatal           0.016           pl5321ha770c72_0    conda-forge
perl-test-warnings        0.031           pl5321ha770c72_0    conda-forge
perl-test2-suite          0.000145        pl5321hdfd78af_0    bioconda
perl-text-format          0.62            pl5321hdfd78af_0    bioconda
perl-time-hires           1.9764          pl5321h031d066_4    bioconda
perl-try-tiny             0.31            pl5321ha770c72_0    conda-forge
perl-types-serialiser     1.01            pl5321hdfd78af_0    bioconda
perl-uri                  5.17            pl5321ha770c72_0    conda-forge
perl-xml-libxml           2.0207          pl5321h661654b_0    bioconda
perl-xml-namespacesupport 1.12            pl5321hd8ed1ab_0    conda-forge
perl-xml-parser           2.44_01         pl5321hc3e0081_1003    conda-forge
perl-xml-sax              1.02            pl5321hd8ed1ab_0    conda-forge
perl-xml-sax-base         1.09            pl5321hd8ed1ab_0    conda-forge
pigz                      2.8                  h2797004_0    conda-forge
piler-cr                  1.06                 h4ac6f70_4    bioconda
pip                       23.3.1             pyhd8ed1ab_0    conda-forge
psutil                    5.9.5           py310h2372a71_1    conda-forge
pycparser                 2.21               pyhd8ed1ab_0    conda-forge
pyhmmer                   0.10.4          py310h4b81fae_0    bioconda
pyrodigal                 2.3.0           py310h4b81fae_1    bioconda
pysocks                   1.7.1              pyha2e5f31_6    conda-forge
python                    3.10.13         hd12c33a_0_cpython    conda-forge
python-isal               1.5.2           py310h2372a71_0    conda-forge
python_abi                3.10                    4_cp310    conda-forge
pyyaml                    6.0.1           py310h2372a71_1    conda-forge
readline                  8.2                  h8228510_1    conda-forge
requests                  2.31.0             pyhd8ed1ab_0    conda-forge
setuptools                68.2.2             pyhd8ed1ab_0    conda-forge
tk                        8.6.13          noxft_h4845f30_101    conda-forge
trnascan-se               2.0.12          pl5321h031d066_0    bioconda
tzdata                    2023c                h71feb2d_0    conda-forge
urllib3                   2.0.7              pyhd8ed1ab_0    conda-forge
wget                      1.20.3               ha35d2d1_1    conda-forge
wheel                     0.41.3             pyhd8ed1ab_0    conda-forge
xopen                     1.8.0           py310hff52083_0    conda-forge
xz                        5.2.6                h166bdaf_0    conda-forge
yaml                      0.2.5                h7f98852_2    conda-forge
zlib                      1.2.13               hd590300_5    conda-forge
zstandard                 0.22.0          py310h1275a96_0    conda-forge
zstd                      1.5.5                hfc55251_0    conda-forge

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oschwengers avatar oschwengers commented on July 23, 2024 1

You're welcome. Glad that it's now working. FYI, upcoming Bakta v1.9.0 will actually use Pyrodigal >= v3.1.0.

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splaisan avatar splaisan commented on July 23, 2024

I reinstalled with conda from scratch once more with conda install -c conda-forge -c bioconda bakta pyrodigal=3.1.0 to hopefully get a better version with Pyrodigal and I do not get a Pyrodigal executable, just pyrodigal.

pyrodigal -V
pyrodigal v3.1.0

my command fails with:

ERROR: Pyrodigal could not be executed! Please make sure Pyrodigal is installed and executable or skip requiring workflow steps via via '--skip-cds'.

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oschwengers avatar oschwengers commented on July 23, 2024

Hi @splaisan,
thanks for reporting. First, to sort things a bit, Bakta actually checks for pyrodigal but uses the canonical tool name Pyrodigal for error messaging. There are more obvious cases like AMRFinderPlus which command is amrfinder.

This having said, I currently do not understand why Bakta fails to recognize Pyrodigal. Could you maybe run Bakta in --debug mode and provide the log file?

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